Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH.
Adhesion to the human intestinal cell is considered as one of the main selection criteria of lactic acid bacteria for probiotic use. The adhesion ability of two Bifidobacteriums strains Bifidobacterium longum BB536 and Bifidobacterium psudocatenulatum G4 was done using HT-29 human epithelium cell...
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| Format: | Article |
| Language: | English English |
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World Academy of Science Engineering and Technology
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12936/1/Adhesion%20properties%20of%20bifidobacterium%20pseudocatenulatum%20G4%20and%20bifidobacterium%20longum%20BB536%20on%20HT.pdf |
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| author | Q.S., Ali A.J., Farid Mohammed Kabeir, Barka Sekawi, Zamberi Mustafa, Shuhaimi Mohd Ghazali, Hasanah Abdul Manap, Yazid |
| author_facet | Q.S., Ali A.J., Farid Mohammed Kabeir, Barka Sekawi, Zamberi Mustafa, Shuhaimi Mohd Ghazali, Hasanah Abdul Manap, Yazid |
| author_sort | Q.S., Ali |
| collection | UPM |
| description | Adhesion to the human intestinal cell is considered as
one of the main selection criteria of lactic acid bacteria for probiotic use. The adhesion ability of two Bifidobacteriums strains Bifidobacterium longum BB536 and Bifidobacterium psudocatenulatum G4 was done using HT-29 human epithelium cell line as in vitro study. Four different level of pH were used 5.6, 5.7, 6.6, and 6.8 with four different times 15, 30, 60, and 120 min. Adhesion was quantified by counting the adhering bacteria after Gram staining. The adhesion of B. longum BB536 was higher than B. psudocatenulatum G4. Both species showed significant different in the adhesion properties at the factors tested. The highest adhesion for both Bifidobacterium was observed at 120 min and the low adhesion was in 15 min. The findings of this study will contribute to the introduction of new effective probiotic strain for future utilization.
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| first_indexed | 2024-03-06T07:26:51Z |
| format | Article |
| id | upm.eprints-12936 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:26:51Z |
| publishDate | 2009 |
| publisher | World Academy of Science Engineering and Technology |
| record_format | dspace |
| spelling | upm.eprints-129362015-10-29T04:21:59Z http://psasir.upm.edu.my/id/eprint/12936/ Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. Q.S., Ali A.J., Farid Mohammed Kabeir, Barka Sekawi, Zamberi Mustafa, Shuhaimi Mohd Ghazali, Hasanah Abdul Manap, Yazid Adhesion to the human intestinal cell is considered as one of the main selection criteria of lactic acid bacteria for probiotic use. The adhesion ability of two Bifidobacteriums strains Bifidobacterium longum BB536 and Bifidobacterium psudocatenulatum G4 was done using HT-29 human epithelium cell line as in vitro study. Four different level of pH were used 5.6, 5.7, 6.6, and 6.8 with four different times 15, 30, 60, and 120 min. Adhesion was quantified by counting the adhering bacteria after Gram staining. The adhesion of B. longum BB536 was higher than B. psudocatenulatum G4. Both species showed significant different in the adhesion properties at the factors tested. The highest adhesion for both Bifidobacterium was observed at 120 min and the low adhesion was in 15 min. The findings of this study will contribute to the introduction of new effective probiotic strain for future utilization. World Academy of Science Engineering and Technology 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12936/1/Adhesion%20properties%20of%20bifidobacterium%20pseudocatenulatum%20G4%20and%20bifidobacterium%20longum%20BB536%20on%20HT.pdf Q.S., Ali and A.J., Farid and Mohammed Kabeir, Barka and Sekawi, Zamberi and Mustafa, Shuhaimi and Mohd Ghazali, Hasanah and Abdul Manap, Yazid (2009) Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. World Academy of Science, Engineering and Technology, 37. pp. 149-153. ISSN 2010-376X; ESSN: 2010-3778 English |
| spellingShingle | Q.S., Ali A.J., Farid Mohammed Kabeir, Barka Sekawi, Zamberi Mustafa, Shuhaimi Mohd Ghazali, Hasanah Abdul Manap, Yazid Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. |
| title | Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. |
| title_full | Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. |
| title_fullStr | Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. |
| title_full_unstemmed | Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. |
| title_short | Adhesion properties of bifidobacterium pseudocatenulatum G4 and bifidobacterium longum BB536 on HT-29 human epithelium cell line at different times and pH. |
| title_sort | adhesion properties of bifidobacterium pseudocatenulatum g4 and bifidobacterium longum bb536 on ht 29 human epithelium cell line at different times and ph |
| url | http://psasir.upm.edu.my/id/eprint/12936/1/Adhesion%20properties%20of%20bifidobacterium%20pseudocatenulatum%20G4%20and%20bifidobacterium%20longum%20BB536%20on%20HT.pdf |
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