Aflatoxin in raw peanut kernels marketed in Malaysia.
The occurrence of af latoxin in eighty-four samples of raw peanut kernels which are randomly collected from Malaysian super- markets was examined. Analysis for af latoxin was performed by solvent extraction and immunoaffinity clean-up followed by the determination using high performance liquid...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
National Laboratories of Foods and Drugs
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/12957/2/Aflatoxin%20in%20raw%20peanut%20kernels%20marketed%20in%20Malaysia.pdf |
| Summary: | The occurrence of af latoxin in eighty-four samples of raw peanut kernels which are randomly
collected from Malaysian super-
markets was examined. Analysis for af latoxin was performed by solvent extraction and
immunoaffinity clean-up followed by the determination using high performance liquid chromatography
equipped with post-column photochemical reactor for enhanced detection and f luorescence detector.
A detection limit of 0.01-0.09 ng/mL and a quantification limit of 0.04-0.30 ng/mL were obtained.
The af latoxin concentrations ranged from not detected to 97.28 ng/g in all samples investigated.
About 78.57% of the samples were contaminated with af latoxin, of which 10.71% exceeded the maximum
tolerable limit of 15 ng/g set by the Codex. Average recoveries of the af latoxin analysis were
acceptable which were in the range of 74.85 ± 8.83% for AFG2 at the concentration of 0.15 ng/mL and
103.91 ± 6.45% for AFB2 at the concentration of 0.15 ng/mL. The average daily intake estimated for
total af la- toxins was 10.69 ng/kg body weight. There was a significant difference (P < 0.05) in
af latoxin content between brands and locations.
|
|---|