An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA...
| Main Authors: | , , , |
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| 格式: | Article |
| 語言: | English |
| 出版: |
Elsevier
2008
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| 在線閱讀: | http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf |
| 總結: | This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA. |
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