An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads
This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf |
| _version_ | 1825945153365344256 |
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| author | Lim, Crystale Siew Ying Tung, Chee Hong Rosli, Rozita Chong, Pei Pei |
| author_facet | Lim, Crystale Siew Ying Tung, Chee Hong Rosli, Rozita Chong, Pei Pei |
| author_sort | Lim, Crystale Siew Ying |
| collection | UPM |
| description | This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA. |
| first_indexed | 2024-03-06T07:27:04Z |
| format | Article |
| id | upm.eprints-13008 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:27:04Z |
| publishDate | 2008 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-130082017-03-28T14:04:09Z http://psasir.upm.edu.my/id/eprint/13008/ An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads Lim, Crystale Siew Ying Tung, Chee Hong Rosli, Rozita Chong, Pei Pei This report describes a modified, cost-effective method of cell wall disruption for the yeast Candida spp., which employs the use of glass beads in a simple sorbitol lysis buffer. This method can be used in conjunction with a commercial RNA or genomic DNA isolation method to obtain high-quality RNA or DNA. Elsevier 2008-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf Lim, Crystale Siew Ying and Tung, Chee Hong and Rosli, Rozita and Chong, Pei Pei (2008) An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads. Journal of Microbiological Methods, 75 (3). pp. 576-578. ISSN 0167-7012; ESSN: 1872-8359 10.1016/j.mimet.2008.07.026 |
| spellingShingle | Lim, Crystale Siew Ying Tung, Chee Hong Rosli, Rozita Chong, Pei Pei An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| title | An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| title_full | An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| title_fullStr | An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| title_full_unstemmed | An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| title_short | An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| title_sort | alternative candida spp cell wall disruption method using a basic sorbitol lysis buffer and glass beads |
| url | http://psasir.upm.edu.my/id/eprint/13008/1/An%20alternative%20Candida%20spp.pdf |
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