Analysis of Sarawak Bario rice diversity using microsatellite markers
Problem statement: There are numerous claims on varieties of Bario rice cultivars in Sarawak. Many of them might be synonyms or homonyms. This preliminary study was to differentiate Bario Rice cultivars using microsatellite molecular markers. Approach: Microsatellite marker panels distributed on all...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Science Publications
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13087/1/ajabssp.2009.298.304.pdf |
| _version_ | 1796968360142962688 |
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| author | Wong, Sie Chuong Yiu, Pang Hung Bong, S. T. W. Lee, Huei Hong Neoh, Phene Pei Nee Amarthalingam, Rajan |
| author_facet | Wong, Sie Chuong Yiu, Pang Hung Bong, S. T. W. Lee, Huei Hong Neoh, Phene Pei Nee Amarthalingam, Rajan |
| author_sort | Wong, Sie Chuong |
| collection | UPM |
| description | Problem statement: There are numerous claims on varieties of Bario rice cultivars in Sarawak. Many of them might be synonyms or homonyms. This preliminary study was to differentiate Bario Rice cultivars using microsatellite molecular markers. Approach: Microsatellite marker panels distributed on all 12 chromosomes on rice genome were employed to study 8 Bario rice cultivars in Sarawak. Results: Current study indicated that the microsatellite panel were able to differentiate the 8 Bario rice cultivars. A total of 31 alleles were generated at 12 polymorphic microsatellite loci among the cultivars using the selected polymorphic SSR primer pairs with an average of 2.6 alleles per locus. Average PIC value obtained was 0.5204. An UPGMA dendrogram based on SSR polymorphism indicated high variation among the rice varieties with the coefficient ranging from 0.16 and 0.92. Genetic diversity determination using cluster analysis showed differentiation of rice cultivars into 2 major groups and several sub-groups. Conclusion: The SSR markers were useful for differentiating the genotypes and assessment of genetic relationships due to its reproducibility and to multiallelic, codominant and informative properties. |
| first_indexed | 2024-03-06T07:27:18Z |
| format | Article |
| id | upm.eprints-13087 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:27:18Z |
| publishDate | 2009 |
| publisher | Science Publications |
| record_format | dspace |
| spelling | upm.eprints-130872017-11-22T02:04:54Z http://psasir.upm.edu.my/id/eprint/13087/ Analysis of Sarawak Bario rice diversity using microsatellite markers Wong, Sie Chuong Yiu, Pang Hung Bong, S. T. W. Lee, Huei Hong Neoh, Phene Pei Nee Amarthalingam, Rajan Problem statement: There are numerous claims on varieties of Bario rice cultivars in Sarawak. Many of them might be synonyms or homonyms. This preliminary study was to differentiate Bario Rice cultivars using microsatellite molecular markers. Approach: Microsatellite marker panels distributed on all 12 chromosomes on rice genome were employed to study 8 Bario rice cultivars in Sarawak. Results: Current study indicated that the microsatellite panel were able to differentiate the 8 Bario rice cultivars. A total of 31 alleles were generated at 12 polymorphic microsatellite loci among the cultivars using the selected polymorphic SSR primer pairs with an average of 2.6 alleles per locus. Average PIC value obtained was 0.5204. An UPGMA dendrogram based on SSR polymorphism indicated high variation among the rice varieties with the coefficient ranging from 0.16 and 0.92. Genetic diversity determination using cluster analysis showed differentiation of rice cultivars into 2 major groups and several sub-groups. Conclusion: The SSR markers were useful for differentiating the genotypes and assessment of genetic relationships due to its reproducibility and to multiallelic, codominant and informative properties. Science Publications 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13087/1/ajabssp.2009.298.304.pdf Wong, Sie Chuong and Yiu, Pang Hung and Bong, S. T. W. and Lee, Huei Hong and Neoh, Phene Pei Nee and Amarthalingam, Rajan (2009) Analysis of Sarawak Bario rice diversity using microsatellite markers. American Journal of Agricultural and Biological Sciences, 4 (4). pp. 298-304. ISSN 1557-4989; ESSN: 1557-4997 http://www.thescipub.com/abstract/?doi=ajabssp.2009.298.304 10.3844/ajabssp.2009.298.304 |
| spellingShingle | Wong, Sie Chuong Yiu, Pang Hung Bong, S. T. W. Lee, Huei Hong Neoh, Phene Pei Nee Amarthalingam, Rajan Analysis of Sarawak Bario rice diversity using microsatellite markers |
| title | Analysis of Sarawak Bario rice diversity using microsatellite markers |
| title_full | Analysis of Sarawak Bario rice diversity using microsatellite markers |
| title_fullStr | Analysis of Sarawak Bario rice diversity using microsatellite markers |
| title_full_unstemmed | Analysis of Sarawak Bario rice diversity using microsatellite markers |
| title_short | Analysis of Sarawak Bario rice diversity using microsatellite markers |
| title_sort | analysis of sarawak bario rice diversity using microsatellite markers |
| url | http://psasir.upm.edu.my/id/eprint/13087/1/ajabssp.2009.298.304.pdf |
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