Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities
The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water, ethanol, ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the che...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Forest Research Institute Malaysia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13182/1/Antioxidant%20and%20anti.pdf |
| _version_ | 1796968379776499712 |
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| author | Pin, Kar Yong Abdullah, Luqman Chuah Ahmad, Abdull Rashih Md. Pisar, Mazura Jamaludin, Fadzureena Subramaniam, Vimala Mat Ali, Rasadah |
| author_facet | Pin, Kar Yong Abdullah, Luqman Chuah Ahmad, Abdull Rashih Md. Pisar, Mazura Jamaludin, Fadzureena Subramaniam, Vimala Mat Ali, Rasadah |
| author_sort | Pin, Kar Yong |
| collection | UPM |
| description | The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water, ethanol, ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to
determine the chemical profiles and concentrations of the active compounds, namely, hydroxychavicol (HC) and eugenol (EU). The antioxidant potential of the extracts was evaluated using two in vitro assays—xanthine/xanthine oxidase superoxide scavenging assay (SOD assay) and 1,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH assay). The anti-inflammatory assays used were hyaluronidase (HYA), xanthine oxidase (XOD) and lipoxygenase (LOX) inhibition assays. The HPLC results revealed that HC and EU were detected in all types of extracts and the concentrations were highest in the water extract. The highest extraction yield was obtained using water. All the extracts were highly active in both antioxidant assays with water extract showing the strongest inhibition. The extracts also exhibited significant inhibition in XOD and LOX assays. The results indicated that the bioactivity of the extracts was related to HC and EU. |
| first_indexed | 2024-03-06T07:27:35Z |
| format | Article |
| id | upm.eprints-13182 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:27:35Z |
| publishDate | 2010 |
| publisher | Forest Research Institute Malaysia |
| record_format | dspace |
| spelling | upm.eprints-131822015-10-29T03:42:19Z http://psasir.upm.edu.my/id/eprint/13182/ Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities Pin, Kar Yong Abdullah, Luqman Chuah Ahmad, Abdull Rashih Md. Pisar, Mazura Jamaludin, Fadzureena Subramaniam, Vimala Mat Ali, Rasadah The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water, ethanol, ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the chemical profiles and concentrations of the active compounds, namely, hydroxychavicol (HC) and eugenol (EU). The antioxidant potential of the extracts was evaluated using two in vitro assays—xanthine/xanthine oxidase superoxide scavenging assay (SOD assay) and 1,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH assay). The anti-inflammatory assays used were hyaluronidase (HYA), xanthine oxidase (XOD) and lipoxygenase (LOX) inhibition assays. The HPLC results revealed that HC and EU were detected in all types of extracts and the concentrations were highest in the water extract. The highest extraction yield was obtained using water. All the extracts were highly active in both antioxidant assays with water extract showing the strongest inhibition. The extracts also exhibited significant inhibition in XOD and LOX assays. The results indicated that the bioactivity of the extracts was related to HC and EU. Forest Research Institute Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13182/1/Antioxidant%20and%20anti.pdf Pin, Kar Yong and Abdullah, Luqman Chuah and Ahmad, Abdull Rashih and Md. Pisar, Mazura and Jamaludin, Fadzureena and Subramaniam, Vimala and Mat Ali, Rasadah (2010) Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities. Journal of Tropical Forest Science, 22 (4). pp. 448-455. ISSN 0128-1283 http://www.frim.gov.my/publication/journal-of-tropical-forest-science-jtfs/ |
| spellingShingle | Pin, Kar Yong Abdullah, Luqman Chuah Ahmad, Abdull Rashih Md. Pisar, Mazura Jamaludin, Fadzureena Subramaniam, Vimala Mat Ali, Rasadah Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities |
| title | Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities |
| title_full | Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities |
| title_fullStr | Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities |
| title_full_unstemmed | Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities |
| title_short | Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities |
| title_sort | antioxidant and anti inflammatory activities of extracts of betel leaves piper betle from solvents with different polarities |
| url | http://psasir.upm.edu.my/id/eprint/13182/1/Antioxidant%20and%20anti.pdf |
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