Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G
The applicability of dye-ligands attached to an expanded bed chromatography quartz base matrix (Streamline™) for the affinity bioseparation of rabbit immunoglobulin G (IgG) was investigated. Reactive Green 5 (RG-5) immobilized onto adsorbent was selected for capturing of rabbit-IgG due to its higher...
| Автори: | , , , , , |
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| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Elsevier
2009
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| Онлайн доступ: | http://psasir.upm.edu.my/id/eprint/13225/1/Application%20of%20dye.pdf |
| _version_ | 1825945195638685696 |
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| author | Wongchuphan, Rattana Tey, Beng Ti Tan, Wen Siang Taip, Farah Saleena Mustapa Kamal, Siti Mazlina Ling, Tau Chuan |
| author_facet | Wongchuphan, Rattana Tey, Beng Ti Tan, Wen Siang Taip, Farah Saleena Mustapa Kamal, Siti Mazlina Ling, Tau Chuan |
| author_sort | Wongchuphan, Rattana |
| collection | UPM |
| description | The applicability of dye-ligands attached to an expanded bed chromatography quartz base matrix (Streamline™) for the affinity bioseparation of rabbit immunoglobulin G (IgG) was investigated. Reactive Green 5 (RG-5) immobilized onto adsorbent was selected for capturing of rabbit-IgG due to its higher binding capacity compared to other dye-ligands possessing similar ligand density. Adsorption parameters such as pH, temperature, ionic strength and initial rabbit-IgG concentration were optimized for the adsorption of rabbit-IgG on the RG-5-immobilized adsorbent. The highest rabbit-IgG adsorption was recorded in pH 7.0, while the maximum binding capacity for BSA was achieved at pH 4.0. The adsorption of rabbit-IgG on RG-5-immobilized adsorbent was declined as the increase of ionic strength. There is no significant influence of temperature against adsorption efficiency of RG-5-immobilized adsorbent for rabbit-IgG. The adsorption phenomenon of rabbit-IgG on RG-5-immobilized adsorbent appeared to follow the Langmuir–Freundlich adsorption isotherm model. The theoretically maximum binding capacity (qm) of RG-5-immobilized adsorbent estimated from this isotherm was 49.3 mg ml−1, which is very close to that obtained experimentally (49.0 mg ml−1). About 50% of bound BSA on RG-5-immobilized adsorbent in binary adsorption system was removed with washing buffer containing 1 M NaCl. |
| first_indexed | 2024-03-06T07:27:43Z |
| format | Article |
| id | upm.eprints-13225 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:27:43Z |
| publishDate | 2009 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-132252015-10-20T02:07:53Z http://psasir.upm.edu.my/id/eprint/13225/ Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G Wongchuphan, Rattana Tey, Beng Ti Tan, Wen Siang Taip, Farah Saleena Mustapa Kamal, Siti Mazlina Ling, Tau Chuan The applicability of dye-ligands attached to an expanded bed chromatography quartz base matrix (Streamline™) for the affinity bioseparation of rabbit immunoglobulin G (IgG) was investigated. Reactive Green 5 (RG-5) immobilized onto adsorbent was selected for capturing of rabbit-IgG due to its higher binding capacity compared to other dye-ligands possessing similar ligand density. Adsorption parameters such as pH, temperature, ionic strength and initial rabbit-IgG concentration were optimized for the adsorption of rabbit-IgG on the RG-5-immobilized adsorbent. The highest rabbit-IgG adsorption was recorded in pH 7.0, while the maximum binding capacity for BSA was achieved at pH 4.0. The adsorption of rabbit-IgG on RG-5-immobilized adsorbent was declined as the increase of ionic strength. There is no significant influence of temperature against adsorption efficiency of RG-5-immobilized adsorbent for rabbit-IgG. The adsorption phenomenon of rabbit-IgG on RG-5-immobilized adsorbent appeared to follow the Langmuir–Freundlich adsorption isotherm model. The theoretically maximum binding capacity (qm) of RG-5-immobilized adsorbent estimated from this isotherm was 49.3 mg ml−1, which is very close to that obtained experimentally (49.0 mg ml−1). About 50% of bound BSA on RG-5-immobilized adsorbent in binary adsorption system was removed with washing buffer containing 1 M NaCl. Elsevier 2009-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13225/1/Application%20of%20dye.pdf Wongchuphan, Rattana and Tey, Beng Ti and Tan, Wen Siang and Taip, Farah Saleena and Mustapa Kamal, Siti Mazlina and Ling, Tau Chuan (2009) Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G. Biochemical Engineering Journal, 45 (3). pp. 232-238. ISSN 1369-703X; ESSN: 1873-295X 10.1016/j.bej.2009.04.003 |
| spellingShingle | Wongchuphan, Rattana Tey, Beng Ti Tan, Wen Siang Taip, Farah Saleena Mustapa Kamal, Siti Mazlina Ling, Tau Chuan Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G |
| title | Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G |
| title_full | Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G |
| title_fullStr | Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G |
| title_full_unstemmed | Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G |
| title_short | Application of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G |
| title_sort | application of dye ligands affinity adsorbent in capturing of rabbit immunoglobulin g |
| url | http://psasir.upm.edu.my/id/eprint/13225/1/Application%20of%20dye.pdf |
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