Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.

Cocoa-specific aroma precursors and methylpyrazines in underfermented cocoa beans obtained from fermentation induced by indigenous carboxypeptidase have been investigated. Fermentation conditions and cocoa bean components were analyzed during 0 to 3 d of fermentation. Underfermented cocoa beans were...

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Main Authors: Selamat, Jinap, Y., Ikrawan, Bakar, Jamilah, Saari, Nazamid, Hanifah Lioe, Nuryani
Format: Article
Language:English
Published: Wiley-Blackwell 2008
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author Selamat, Jinap
Y., Ikrawan
Bakar, Jamilah
Saari, Nazamid
Hanifah Lioe, Nuryani
author_facet Selamat, Jinap
Y., Ikrawan
Bakar, Jamilah
Saari, Nazamid
Hanifah Lioe, Nuryani
author_sort Selamat, Jinap
collection UPM
description Cocoa-specific aroma precursors and methylpyrazines in underfermented cocoa beans obtained from fermentation induced by indigenous carboxypeptidase have been investigated. Fermentation conditions and cocoa bean components were analyzed during 0 to 3 d of fermentation. Underfermented cocoa beans were characterized as having hydrophilic peptides and free hydrophobic amino acids much higher than unfermented ones. These 2 key components of cocoa aroma precursors may be produced from the breakdown of proteins and polypeptides by endogenous carboxypeptidase during the fermentation process. The enzyme was activated during fermentation. Polypeptides of 47, 31, and 19 kDa were observed in the samples throughout the 3-d fermentation period; however, only the first 2 polypeptides were remarkably reduced during fermentation. Since the 1st day of fermentation, underfermented cocoa beans contained methylpyrazines, a dominant group of cocoa-specific aroma. This might be due to microbial activities during fermentation, observed through a decrease of pH value and an increase of temperature of cocoa beans. The concentration of tetramethylpyrazines was significantly increased during the 3 d of fermentation. This may increase the cocoa-specific flavor to the beans.
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spelling upm.eprints-132722013-12-10T04:09:02Z http://psasir.upm.edu.my/id/eprint/13272/ Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase. Selamat, Jinap Y., Ikrawan Bakar, Jamilah Saari, Nazamid Hanifah Lioe, Nuryani Cocoa-specific aroma precursors and methylpyrazines in underfermented cocoa beans obtained from fermentation induced by indigenous carboxypeptidase have been investigated. Fermentation conditions and cocoa bean components were analyzed during 0 to 3 d of fermentation. Underfermented cocoa beans were characterized as having hydrophilic peptides and free hydrophobic amino acids much higher than unfermented ones. These 2 key components of cocoa aroma precursors may be produced from the breakdown of proteins and polypeptides by endogenous carboxypeptidase during the fermentation process. The enzyme was activated during fermentation. Polypeptides of 47, 31, and 19 kDa were observed in the samples throughout the 3-d fermentation period; however, only the first 2 polypeptides were remarkably reduced during fermentation. Since the 1st day of fermentation, underfermented cocoa beans contained methylpyrazines, a dominant group of cocoa-specific aroma. This might be due to microbial activities during fermentation, observed through a decrease of pH value and an increase of temperature of cocoa beans. The concentration of tetramethylpyrazines was significantly increased during the 3 d of fermentation. This may increase the cocoa-specific flavor to the beans. Wiley-Blackwell 2008 Article PeerReviewed Selamat, Jinap and Y., Ikrawan and Bakar, Jamilah and Saari, Nazamid and Hanifah Lioe, Nuryani (2008) Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase. Journal of Food Science, 73 (7). pp. 142-147. ISSN 0022-1147; ESSN: 1750-3841 10.1111/j.1750-3841.2008.00858.x English
spellingShingle Selamat, Jinap
Y., Ikrawan
Bakar, Jamilah
Saari, Nazamid
Hanifah Lioe, Nuryani
Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.
title Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.
title_full Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.
title_fullStr Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.
title_full_unstemmed Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.
title_short Aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase.
title_sort aroma precursors and methylpyrazines in underfermented cocoa beans induced by endogenous carboxypeptidase
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AT yikrawan aromaprecursorsandmethylpyrazinesinunderfermentedcocoabeansinducedbyendogenouscarboxypeptidase
AT bakarjamilah aromaprecursorsandmethylpyrazinesinunderfermentedcocoabeansinducedbyendogenouscarboxypeptidase
AT saarinazamid aromaprecursorsandmethylpyrazinesinunderfermentedcocoabeansinducedbyendogenouscarboxypeptidase
AT hanifahlioenuryani aromaprecursorsandmethylpyrazinesinunderfermentedcocoabeansinducedbyendogenouscarboxypeptidase