Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes
Hairy roots of Solenostemon scutellarioides were induced by inoculation of leaf explants with Agrobacterium rhizogenes strains TR 105, LBA 9402, 8196 and ATCC 15834. These strains showed different abilities to induce hairy root formation on the leaf explants. Assessment of the plant’s susceptibility...
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Format: | Article |
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Academic Journals
2009
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Online Access: | http://psasir.upm.edu.my/id/eprint/13302/1/13302.pdf |
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author | Mohd. Saleh, Norihan Thuc, Le Vinh |
author_facet | Mohd. Saleh, Norihan Thuc, Le Vinh |
author_sort | Mohd. Saleh, Norihan |
collection | UPM |
description | Hairy roots of Solenostemon scutellarioides were induced by inoculation of leaf explants with Agrobacterium rhizogenes strains TR 105, LBA 9402, 8196 and ATCC 15834. These strains showed different abilities to induce hairy root formation on the leaf explants. Assessment of the plant’s susceptibility to the different strains of A. rhizogenes showed that strains ATCC 15834, TR 105, LBA 9402, and 8196 produced 56.3, 25.5, 21.5 and 13.8% transformation efficiencies, respectively. Acetosyringone was found to be useful for the enhancement of hairy roots formation in S. scutellarioides. |
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format | Article |
id | upm.eprints-13302 |
institution | Universiti Putra Malaysia |
language | English |
last_indexed | 2024-03-06T07:27:57Z |
publishDate | 2009 |
publisher | Academic Journals |
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spelling | upm.eprints-133022017-11-14T09:53:28Z http://psasir.upm.edu.my/id/eprint/13302/ Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes Mohd. Saleh, Norihan Thuc, Le Vinh Hairy roots of Solenostemon scutellarioides were induced by inoculation of leaf explants with Agrobacterium rhizogenes strains TR 105, LBA 9402, 8196 and ATCC 15834. These strains showed different abilities to induce hairy root formation on the leaf explants. Assessment of the plant’s susceptibility to the different strains of A. rhizogenes showed that strains ATCC 15834, TR 105, LBA 9402, and 8196 produced 56.3, 25.5, 21.5 and 13.8% transformation efficiencies, respectively. Acetosyringone was found to be useful for the enhancement of hairy roots formation in S. scutellarioides. Academic Journals 2009-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13302/1/13302.pdf Mohd. Saleh, Norihan and Thuc, Le Vinh (2009) Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes. African Journal of Biotechnology, 8 (15). art. no. F1D1EF16545. pp. 3519-3523. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/F1D1EF16545 |
spellingShingle | Mohd. Saleh, Norihan Thuc, Le Vinh Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes |
title | Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes |
title_full | Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes |
title_fullStr | Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes |
title_full_unstemmed | Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes |
title_short | Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes |
title_sort | assessment of hairy roots induction in solenostemon scutellarioides leaves by different strains of agrobacterium rhizogenes |
url | http://psasir.upm.edu.my/id/eprint/13302/1/13302.pdf |
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