Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients.
Multidrug resistance (MDR) is believed to be responsible for poor response of patients towards chemotherapy particularly patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The best-characterized resistance mechanism is the one mediated by permeability-glycoprotein (P-...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society of Pathologists
2008
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/13305/1/Assessment%20of%20P.pdf |
| _version_ | 1825945211124056064 |
|---|---|
| author | Nordin, Fazlina Abdullah, Maha Abdul Latiff , Zarina Alias, Hamidah Syed Zakaria, Syed Zulkifli Cheong, Soon Keng Othman, Ainoon Jamal, Rahman Noor Hamidah, |
| author_facet | Nordin, Fazlina Abdullah, Maha Abdul Latiff , Zarina Alias, Hamidah Syed Zakaria, Syed Zulkifli Cheong, Soon Keng Othman, Ainoon Jamal, Rahman Noor Hamidah, |
| author_sort | Nordin, Fazlina |
| collection | UPM |
| description | Multidrug resistance (MDR) is believed to be responsible for poor response of patients towards chemotherapy particularly patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The best-characterized resistance mechanism is the one mediated by permeability-glycoprotein (P-gp) encoded by MDR1 gene, which is responsible for drug efflux. We studied P-gp and multidrug resistance-associated protein 1 (MRP1) expression and functional activities in 43 newly diagnosed acute leukemia cases (19 paediatric ALL cases and 24 adult AML cases). The expression and functional activities were examined using flow cytometry and MultiDrugQuant assay kit (involving calcein AM uptake and efflux). P-gp and MRP1 expression and its functional activities were observed in 68.4% of paediatric ALL. In adult AML cases, all cases expressed MRP1 and its functional activities but only 58.3% were positive for P-gp and its functional activities. We were able to show a significant correlation between the expression of the multidrug resistant protein (P-gp and MRP1) and their functional activity in adult AML and paediatric ALL samples. |
| first_indexed | 2024-03-06T07:27:58Z |
| format | Article |
| id | upm.eprints-13305 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:27:58Z |
| publishDate | 2008 |
| publisher | Malaysian Society of Pathologists |
| record_format | dspace |
| spelling | upm.eprints-133052015-12-22T02:17:20Z http://psasir.upm.edu.my/id/eprint/13305/ Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. Nordin, Fazlina Abdullah, Maha Abdul Latiff , Zarina Alias, Hamidah Syed Zakaria, Syed Zulkifli Cheong, Soon Keng Othman, Ainoon Jamal, Rahman Noor Hamidah, Multidrug resistance (MDR) is believed to be responsible for poor response of patients towards chemotherapy particularly patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The best-characterized resistance mechanism is the one mediated by permeability-glycoprotein (P-gp) encoded by MDR1 gene, which is responsible for drug efflux. We studied P-gp and multidrug resistance-associated protein 1 (MRP1) expression and functional activities in 43 newly diagnosed acute leukemia cases (19 paediatric ALL cases and 24 adult AML cases). The expression and functional activities were examined using flow cytometry and MultiDrugQuant assay kit (involving calcein AM uptake and efflux). P-gp and MRP1 expression and its functional activities were observed in 68.4% of paediatric ALL. In adult AML cases, all cases expressed MRP1 and its functional activities but only 58.3% were positive for P-gp and its functional activities. We were able to show a significant correlation between the expression of the multidrug resistant protein (P-gp and MRP1) and their functional activity in adult AML and paediatric ALL samples. Malaysian Society of Pathologists 2008-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13305/1/Assessment%20of%20P.pdf Nordin, Fazlina and Abdullah, Maha and Abdul Latiff , Zarina and Alias, Hamidah and Syed Zakaria, Syed Zulkifli and Cheong, Soon Keng and Othman, Ainoon and Jamal, Rahman and Noor Hamidah, (2008) Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. Malaysian Journal of Pathology, 30 (2). pp. 87-93. ISSN 0126-8635 http://www.mjpath.org.my/2008.2/index.html |
| spellingShingle | Nordin, Fazlina Abdullah, Maha Abdul Latiff , Zarina Alias, Hamidah Syed Zakaria, Syed Zulkifli Cheong, Soon Keng Othman, Ainoon Jamal, Rahman Noor Hamidah, Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. |
| title | Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. |
| title_full | Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. |
| title_fullStr | Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. |
| title_full_unstemmed | Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. |
| title_short | Assessment of P-gp and MRP1 activities using MultiDrugQuant Assay Kit : a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients. |
| title_sort | assessment of p gp and mrp1 activities using multidrugquant assay kit a preliminary study of correlation between protein expressions and its functional activities in newly diagnosed acute leukaemia patients |
| url | http://psasir.upm.edu.my/id/eprint/13305/1/Assessment%20of%20P.pdf |
| work_keys_str_mv | AT nordinfazlina assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT abdullahmaha assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT abdullatiffzarina assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT aliashamidah assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT syedzakariasyedzulkifli assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT cheongsoonkeng assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT othmanainoon assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT jamalrahman assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients AT noorhamidah assessmentofpgpandmrp1activitiesusingmultidrugquantassaykitapreliminarystudyofcorrelationbetweenproteinexpressionsanditsfunctionalactivitiesinnewlydiagnosedacuteleukaemiapatients |