Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7.
In a previous communication we showed that atrovirinone, a 1,4-benzoquinone isolated from the roots of Garcinia atroviridis, was able to inhibit several major proinflammatory mediators of inflammation. In this report we show that atrovirinone inhibits NO and PGE2 synthesis through inhibition of iNOS...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13323/1/Atrovirinone%20inhibits%20proinflammatory%20mediator%20synthesis%20through%20disruption%20of%20NF.pdf |
| _version_ | 1825945214606376960 |
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| author | Israf Ali, Daud Ahmad Tham, Chau Ling Ahmad, Syahida Lajis, Md. Nordin Sulaiman, Mohd Roslan Mohamad, Azam Shah Zakaria, Zainul Amiruddin |
| author_facet | Israf Ali, Daud Ahmad Tham, Chau Ling Ahmad, Syahida Lajis, Md. Nordin Sulaiman, Mohd Roslan Mohamad, Azam Shah Zakaria, Zainul Amiruddin |
| author_sort | Israf Ali, Daud Ahmad |
| collection | UPM |
| description | In a previous communication we showed that atrovirinone, a 1,4-benzoquinone isolated from the roots of Garcinia atroviridis, was able to inhibit several major proinflammatory mediators of inflammation. In this report we show that atrovirinone inhibits NO and PGE2 synthesis through inhibition of iNOS and COX-2 expression. We also show that atrovirinone inhibits the secretion of IL-1β and IL-6 in a dose dependent fashion whereas the secretion of IL-10, the anti-inflammatory cytokine, was enhanced. Subsequently we determined that the inhibition of proinflammatory cytokine synthesis and inducible enzyme expression was due to a dose-dependent inhibition of phosphorylation of p38 and ERK1/2. We also showed that atrovirinone prevented phosphorylation of I-κBα, which resulted in a reduction of p65NF-κB nuclear translocation as demonstrated by expression analysis. We conclude that atrovirinone is a potential anti-inflammatory drug lead that targets both the MAPK and NF-κB pathway. |
| first_indexed | 2024-03-06T07:28:01Z |
| format | Article |
| id | upm.eprints-13323 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:28:01Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-133232015-09-28T01:32:08Z http://psasir.upm.edu.my/id/eprint/13323/ Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. Israf Ali, Daud Ahmad Tham, Chau Ling Ahmad, Syahida Lajis, Md. Nordin Sulaiman, Mohd Roslan Mohamad, Azam Shah Zakaria, Zainul Amiruddin In a previous communication we showed that atrovirinone, a 1,4-benzoquinone isolated from the roots of Garcinia atroviridis, was able to inhibit several major proinflammatory mediators of inflammation. In this report we show that atrovirinone inhibits NO and PGE2 synthesis through inhibition of iNOS and COX-2 expression. We also show that atrovirinone inhibits the secretion of IL-1β and IL-6 in a dose dependent fashion whereas the secretion of IL-10, the anti-inflammatory cytokine, was enhanced. Subsequently we determined that the inhibition of proinflammatory cytokine synthesis and inducible enzyme expression was due to a dose-dependent inhibition of phosphorylation of p38 and ERK1/2. We also showed that atrovirinone prevented phosphorylation of I-κBα, which resulted in a reduction of p65NF-κB nuclear translocation as demonstrated by expression analysis. We conclude that atrovirinone is a potential anti-inflammatory drug lead that targets both the MAPK and NF-κB pathway. Elsevier 2010-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13323/1/Atrovirinone%20inhibits%20proinflammatory%20mediator%20synthesis%20through%20disruption%20of%20NF.pdf Israf Ali, Daud Ahmad and Tham, Chau Ling and Ahmad, Syahida and Lajis, Md. Nordin and Sulaiman, Mohd Roslan and Mohamad, Azam Shah and Zakaria, Zainul Amiruddin (2010) Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. Phytomedicine, 17 (10). pp. 732-739. ISSN 0944-7113; ESSN: 1618-095X http://www.phytomedicinejournal.com/ 10.1016/j.phymed.2010.02.006 English |
| spellingShingle | Israf Ali, Daud Ahmad Tham, Chau Ling Ahmad, Syahida Lajis, Md. Nordin Sulaiman, Mohd Roslan Mohamad, Azam Shah Zakaria, Zainul Amiruddin Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. |
| title | Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. |
| title_full | Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. |
| title_fullStr | Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. |
| title_full_unstemmed | Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. |
| title_short | Atrovirinone inhibits proinflammatory mediator synthesis through disruption of NF-kappaB nuclear translocation and MAPK phosphorylation in the murine monocytic macrophage RAW 264.7. |
| title_sort | atrovirinone inhibits proinflammatory mediator synthesis through disruption of nf kappab nuclear translocation and mapk phosphorylation in the murine monocytic macrophage raw 264 7 |
| url | http://psasir.upm.edu.my/id/eprint/13323/1/Atrovirinone%20inhibits%20proinflammatory%20mediator%20synthesis%20through%20disruption%20of%20NF.pdf |
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