Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease
Immune responses in association with body weight performance and hemato-biochemical constituents might influence the Newcastle disease (ND) vaccination by dietary phytase supplementation (Enterobacter sakazakii ASUA273). The objective of the study was to determine the effects of bacterial phytase su...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary Association Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/134/1/2.%20EFFECTS%20OF%20LOCALLY%20PRODUCED%20BACTERIAL%20PHYTASE%20ON%20HUMORAL%20IMMUNITY%2C%20LIVE%20BODY.pdf |
| _version_ | 1825943151577137152 |
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| author | Islam, R. Ideris, Aini Kasim, Azhar Omar, Abdul Rahman Meor Hussin, Anis Shobirin Farhana Yasmin, Akter, Yeasmin |
| author_facet | Islam, R. Ideris, Aini Kasim, Azhar Omar, Abdul Rahman Meor Hussin, Anis Shobirin Farhana Yasmin, Akter, Yeasmin |
| author_sort | Islam, R. |
| collection | UPM |
| description | Immune responses in association with body weight performance and hemato-biochemical constituents might influence the Newcastle disease (ND) vaccination by dietary phytase supplementation (Enterobacter sakazakii ASUA273). The objective of the study was to determine the effects of bacterial phytase supplementation on humoral immunity in association with live body weight and blood characteristics of broilers. Male-day-old Cobb broilers (n=180) were assigned into four phytase treatments (0, 500, 1000
and 1500 FTU/kg-1 of diet) with 12 cages comprised of 3 replicates and each cage contained 15 birds. Birds were maintained on formulated basal diet based with available
phosphorus (0.19%) that lasted up to six weeks in feed. Birds were vaccinated with a live ND vaccine at day-old and 21 day-old. Two birds were randomly selected from each treatment weekly. Specific antibody to ND, non-specific immunoglobins (IgM, IgG, and IgA) and live body weight were measured weekly. At the end of experiment, the
complete haemato-biochemical constituents were determined. Data of humoral immunity with live body weight and haemato-
biochemical values were analysed based on factorial arrangement (treatments×weeks) of completely randomized design (CRD), respectively. Response of the humoral immunity shown that although serum-antibody of ND, IgM, and IgG levels were not improved, the mucosal IgA contents were increased with the increasing phytase doses. The live
body weights of broilers were significantly increased (P<0.05) to the graded levels of phytase supplementation throughout the experimental period. Cumulative
effects of mucosal IgA contents and live body weights of broilers showed significant (P<0.05) interaction between the effects of phytase levels and effects of weeks. Overall, phytase dose at 1500 FTU/kg-1 of diet over the age of 6 weeks-old showed the best performance.
Conversely, no significant, sequential, and consistent treatment effects were observed on hemato-biochemical
constituents in broiler chickens. It is therefore, concluded that the efficacy of this local phytase was flourished in respective to mucosal IgA contents and live body weights of broilers. |
| first_indexed | 2024-03-06T06:52:22Z |
| format | Article |
| id | upm.eprints-134 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T06:52:22Z |
| publishDate | 2014 |
| publisher | Veterinary Association Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1342015-09-11T04:34:18Z http://psasir.upm.edu.my/id/eprint/134/ Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease Islam, R. Ideris, Aini Kasim, Azhar Omar, Abdul Rahman Meor Hussin, Anis Shobirin Farhana Yasmin, Akter, Yeasmin Immune responses in association with body weight performance and hemato-biochemical constituents might influence the Newcastle disease (ND) vaccination by dietary phytase supplementation (Enterobacter sakazakii ASUA273). The objective of the study was to determine the effects of bacterial phytase supplementation on humoral immunity in association with live body weight and blood characteristics of broilers. Male-day-old Cobb broilers (n=180) were assigned into four phytase treatments (0, 500, 1000 and 1500 FTU/kg-1 of diet) with 12 cages comprised of 3 replicates and each cage contained 15 birds. Birds were maintained on formulated basal diet based with available phosphorus (0.19%) that lasted up to six weeks in feed. Birds were vaccinated with a live ND vaccine at day-old and 21 day-old. Two birds were randomly selected from each treatment weekly. Specific antibody to ND, non-specific immunoglobins (IgM, IgG, and IgA) and live body weight were measured weekly. At the end of experiment, the complete haemato-biochemical constituents were determined. Data of humoral immunity with live body weight and haemato- biochemical values were analysed based on factorial arrangement (treatments×weeks) of completely randomized design (CRD), respectively. Response of the humoral immunity shown that although serum-antibody of ND, IgM, and IgG levels were not improved, the mucosal IgA contents were increased with the increasing phytase doses. The live body weights of broilers were significantly increased (P<0.05) to the graded levels of phytase supplementation throughout the experimental period. Cumulative effects of mucosal IgA contents and live body weights of broilers showed significant (P<0.05) interaction between the effects of phytase levels and effects of weeks. Overall, phytase dose at 1500 FTU/kg-1 of diet over the age of 6 weeks-old showed the best performance. Conversely, no significant, sequential, and consistent treatment effects were observed on hemato-biochemical constituents in broiler chickens. It is therefore, concluded that the efficacy of this local phytase was flourished in respective to mucosal IgA contents and live body weights of broilers. Veterinary Association Malaysia 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/134/1/2.%20EFFECTS%20OF%20LOCALLY%20PRODUCED%20BACTERIAL%20PHYTASE%20ON%20HUMORAL%20IMMUNITY%2C%20LIVE%20BODY.pdf Islam, R. and Ideris, Aini and Kasim, Azhar and Omar, Abdul Rahman and Meor Hussin, Anis Shobirin and Farhana Yasmin, and Akter, Yeasmin (2014) Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease. Jurnal Veterinar Malaysia, 26 (1&2). pp. 8-16. ISSN 9128-2506 |
| spellingShingle | Islam, R. Ideris, Aini Kasim, Azhar Omar, Abdul Rahman Meor Hussin, Anis Shobirin Farhana Yasmin, Akter, Yeasmin Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease |
| title | Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease |
| title_full | Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease |
| title_fullStr | Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease |
| title_full_unstemmed | Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease |
| title_short | Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease |
| title_sort | effects of locally produced bacterial phytase on humoral immunity live body weight and blood characteristics in broilers vaccinated against newcastle disease |
| url | http://psasir.upm.edu.my/id/eprint/134/1/2.%20EFFECTS%20OF%20LOCALLY%20PRODUCED%20BACTERIAL%20PHYTASE%20ON%20HUMORAL%20IMMUNITY%2C%20LIVE%20BODY.pdf |
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