Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas).
Current practice of indiscriminate and illegal felling of 'karas' trees (Aquilaria malaccensis) to extract 'gaharu' in the wild have caused the population to dwindle drastically. 'Gaharu' formed as a result of natural defense mechanism in trees, which unfortunately happ...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
Forestry Department Peninsular Malaysia
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13609/1/Cloning%20of%20Phenylalanine%20ammonia.pdf |
| _version_ | 1825945269035859968 |
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| author | Mohamed, Rozi Wong, M. T. |
| author_facet | Mohamed, Rozi Wong, M. T. |
| author_sort | Mohamed, Rozi |
| collection | UPM |
| description | Current practice of indiscriminate and illegal felling of 'karas' trees (Aquilaria malaccensis) to extract 'gaharu' in the wild have caused the population to dwindle drastically. 'Gaharu' formed as a result of natural defense mechanism in trees, which unfortunately happens over a long period of time. To better understand natural processes of gaharu-making, we sought to study the roles of defense genes in 'karas' by cloning a gene that synthesizes secondary compounds. The phenylalanine ammonia-lyase (PAL) gene encodes an enzyme in the metabolism of phenylpropanoid compounds, which are produced in response to stress, such as damage by wounding and pathogen attack. We report a cloned PAL gene fragment from A. malaccensis using the polymerase chain reaction (PCR) technique. The fragment was amplified from genomic DNA of a wounded 2-year old tree and cloned into the pGEM-T Easy Vector. The cloned fragment had a length of 610bp and an uninterrupted open reading frame of 201 amino acids. When searched against the Genebank database using BLAST tools, the cloned fragment shared 92% sequence similarity at amino acid level to known PAL proteins. This work will serve as a foundation in identifying important genes for triggering 'gaharu' synthesis. |
| first_indexed | 2024-03-06T07:28:52Z |
| format | Article |
| id | upm.eprints-13609 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:28:52Z |
| publishDate | 2009 |
| publisher | Forestry Department Peninsular Malaysia |
| record_format | dspace |
| spelling | upm.eprints-136092015-10-22T06:05:40Z http://psasir.upm.edu.my/id/eprint/13609/ Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). Mohamed, Rozi Wong, M. T. Current practice of indiscriminate and illegal felling of 'karas' trees (Aquilaria malaccensis) to extract 'gaharu' in the wild have caused the population to dwindle drastically. 'Gaharu' formed as a result of natural defense mechanism in trees, which unfortunately happens over a long period of time. To better understand natural processes of gaharu-making, we sought to study the roles of defense genes in 'karas' by cloning a gene that synthesizes secondary compounds. The phenylalanine ammonia-lyase (PAL) gene encodes an enzyme in the metabolism of phenylpropanoid compounds, which are produced in response to stress, such as damage by wounding and pathogen attack. We report a cloned PAL gene fragment from A. malaccensis using the polymerase chain reaction (PCR) technique. The fragment was amplified from genomic DNA of a wounded 2-year old tree and cloned into the pGEM-T Easy Vector. The cloned fragment had a length of 610bp and an uninterrupted open reading frame of 201 amino acids. When searched against the Genebank database using BLAST tools, the cloned fragment shared 92% sequence similarity at amino acid level to known PAL proteins. This work will serve as a foundation in identifying important genes for triggering 'gaharu' synthesis. Forestry Department Peninsular Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13609/1/Cloning%20of%20Phenylalanine%20ammonia.pdf Mohamed, Rozi and Wong, M. T. (2009) Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). The Malaysian Forester, 72 (1). pp. 45-51. ISSN 0302-2935 English |
| spellingShingle | Mohamed, Rozi Wong, M. T. Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). |
| title | Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). |
| title_full | Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). |
| title_fullStr | Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). |
| title_full_unstemmed | Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). |
| title_short | Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas). |
| title_sort | cloning of phenylalanine ammonia lyase pal gene fragment from aquilaria malaccensis lam karas |
| url | http://psasir.upm.edu.my/id/eprint/13609/1/Cloning%20of%20Phenylalanine%20ammonia.pdf |
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