Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer
Eleven potential polyhydroxybutyrates (PHB) producers were successfully screened from treated palm oil waste water using acetate as sole carbon and energy sources and on nitrogen limiting media with Nile Blue A dye to screen for PHB accumulation. Orange fluorescence color, produced upon binding of t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13630/1/13630.pdf |
| _version_ | 1796968475746369536 |
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| author | Zakaria, Mohd Rafein Abd. Aziz, Suraini Ariffin, Hidayah Abdul Rahman, Nor'aini Phang, Lai Yee Hassan, Mohd Ali |
| author_facet | Zakaria, Mohd Rafein Abd. Aziz, Suraini Ariffin, Hidayah Abdul Rahman, Nor'aini Phang, Lai Yee Hassan, Mohd Ali |
| author_sort | Zakaria, Mohd Rafein |
| collection | UPM |
| description | Eleven potential polyhydroxybutyrates (PHB) producers were successfully screened from treated palm oil waste water using acetate as sole carbon and energy sources and on nitrogen limiting media with Nile Blue A dye to screen for PHB accumulation. Orange fluorescence color, produced upon binding of the dye to polymer granules in the cell, was detected when directly exposed under ultra violet light and
it was further confirmed using fluorescence microscopes. Positive isolate designated as PHB5 gave the highest PHB content of 44% (g PHB/g CDW) in shake flask experiment. The metabolic properties of the bacteria was characterized using BIOLOG program and this bacterium was identified belongs to the genus Comamonas sp. Based on the peaks detected from 1H-NMR analysis, it is clearly indicated that
the polyhydroxyalkanoates (PHA) produced from the bacteria was poly-(hydrobutyrate-cohydroxyvalarate)(PHBV) when the bacterium was supplied with 10 g/L acids mixture (acetic 5 :
propionic 3 : butyric 2) derived from palm oil mill effluent (POME) treatment. |
| first_indexed | 2024-03-06T07:28:55Z |
| format | Article |
| id | upm.eprints-13630 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:28:55Z |
| publishDate | 2008 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-136302016-04-27T02:55:38Z http://psasir.upm.edu.my/id/eprint/13630/ Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer Zakaria, Mohd Rafein Abd. Aziz, Suraini Ariffin, Hidayah Abdul Rahman, Nor'aini Phang, Lai Yee Hassan, Mohd Ali Eleven potential polyhydroxybutyrates (PHB) producers were successfully screened from treated palm oil waste water using acetate as sole carbon and energy sources and on nitrogen limiting media with Nile Blue A dye to screen for PHB accumulation. Orange fluorescence color, produced upon binding of the dye to polymer granules in the cell, was detected when directly exposed under ultra violet light and it was further confirmed using fluorescence microscopes. Positive isolate designated as PHB5 gave the highest PHB content of 44% (g PHB/g CDW) in shake flask experiment. The metabolic properties of the bacteria was characterized using BIOLOG program and this bacterium was identified belongs to the genus Comamonas sp. Based on the peaks detected from 1H-NMR analysis, it is clearly indicated that the polyhydroxyalkanoates (PHA) produced from the bacteria was poly-(hydrobutyrate-cohydroxyvalarate)(PHBV) when the bacterium was supplied with 10 g/L acids mixture (acetic 5 : propionic 3 : butyric 2) derived from palm oil mill effluent (POME) treatment. Academic Journals 2008-11-19 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13630/1/13630.pdf Zakaria, Mohd Rafein and Abd. Aziz, Suraini and Ariffin, Hidayah and Abdul Rahman, Nor'aini and Phang, Lai Yee and Hassan, Mohd Ali (2008) Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer. African Journal of Biotechnology, 7 (22). art. no. 1DD67F98324. pp. 4118-4121. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/1DD67F98324 |
| spellingShingle | Zakaria, Mohd Rafein Abd. Aziz, Suraini Ariffin, Hidayah Abdul Rahman, Nor'aini Phang, Lai Yee Hassan, Mohd Ali Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer |
| title | Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer |
| title_full | Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer |
| title_fullStr | Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer |
| title_full_unstemmed | Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer |
| title_short | Comamonas sp. EB172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate (PHA) producer |
| title_sort | comamonas sp eb172 isolated from digester treating palm oil mill effluent as potential polyhydroxyalkanoate pha producer |
| url | http://psasir.upm.edu.my/id/eprint/13630/1/13630.pdf |
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