Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm
The construction of transformation vectors carrying bioplastic biosynthetic genes driven by constitutive and oil palm mesocarp-specific promoters was completed. Four planned transformation vectors were produced. The poly-3-hydroxybutyrate (PHB) producing constructs carried the phbA, phbB mid phbC ge...
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Palm Oil Board
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13759/1/Construction%20of%20PHB%20and%20PHBV%20transformation%20vectors%20for%20bioplastics%20production%20in%20oil%20palm.pdf |
| _version_ | 1825945297663033344 |
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| author | Mat Yunus, Abdul Masani Ho, Chai Ling Ghulam Kadir, Ahmad Parveez |
| author_facet | Mat Yunus, Abdul Masani Ho, Chai Ling Ghulam Kadir, Ahmad Parveez |
| author_sort | Mat Yunus, Abdul Masani |
| collection | UPM |
| description | The construction of transformation vectors carrying bioplastic biosynthetic genes driven by constitutive and oil palm mesocarp-specific promoters was completed. Four planned transformation vectors were produced. The poly-3-hydroxybutyrate (PHB) producing constructs carried the phbA, phbB mid phbC genes, while the polyhyroxybutyrate-co-valerate (PHBV) producing constructs carried the bktB, phbB, phbC and tdcB genes. Each of these genes was fused with the transit peptide (Tp) of the oil palm acyl-carrier-protein (ACP) for targeting into the plastids of plant cells. All vectors carry the phosphinothricin acetyltransferase gene (bar) driven by an ubiquitin promoter as plant selectable marker. The matrix attachment region from tobacco (RB7MAR) was also included for stabilization of the transgene expression and to minimize the gene silencing due to positional effects. All constructs were verified by restriction analysis, polymerase chain reaction (PCR) and DNA sequencing. |
| first_indexed | 2024-03-06T07:29:18Z |
| format | Article |
| id | upm.eprints-13759 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:29:18Z |
| publishDate | 2008 |
| publisher | Malaysian Palm Oil Board |
| record_format | dspace |
| spelling | upm.eprints-137592019-10-22T01:21:29Z http://psasir.upm.edu.my/id/eprint/13759/ Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm Mat Yunus, Abdul Masani Ho, Chai Ling Ghulam Kadir, Ahmad Parveez The construction of transformation vectors carrying bioplastic biosynthetic genes driven by constitutive and oil palm mesocarp-specific promoters was completed. Four planned transformation vectors were produced. The poly-3-hydroxybutyrate (PHB) producing constructs carried the phbA, phbB mid phbC genes, while the polyhyroxybutyrate-co-valerate (PHBV) producing constructs carried the bktB, phbB, phbC and tdcB genes. Each of these genes was fused with the transit peptide (Tp) of the oil palm acyl-carrier-protein (ACP) for targeting into the plastids of plant cells. All vectors carry the phosphinothricin acetyltransferase gene (bar) driven by an ubiquitin promoter as plant selectable marker. The matrix attachment region from tobacco (RB7MAR) was also included for stabilization of the transgene expression and to minimize the gene silencing due to positional effects. All constructs were verified by restriction analysis, polymerase chain reaction (PCR) and DNA sequencing. Malaysian Palm Oil Board 2008 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/13759/1/Construction%20of%20PHB%20and%20PHBV%20transformation%20vectors%20for%20bioplastics%20production%20in%20oil%20palm.pdf Mat Yunus, Abdul Masani and Ho, Chai Ling and Ghulam Kadir, Ahmad Parveez (2008) Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm. Journal of Oil Palm Research (spec. July). pp. 37-55. ISSN 1511-2780 http://jopr.mpob.gov.my/construction-of-phb-and-phbv-transformation-vectors-for-bioplastics-production-in-oil-palm/ |
| spellingShingle | Mat Yunus, Abdul Masani Ho, Chai Ling Ghulam Kadir, Ahmad Parveez Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm |
| title | Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm |
| title_full | Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm |
| title_fullStr | Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm |
| title_full_unstemmed | Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm |
| title_short | Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm |
| title_sort | construction of phb and phbv transformation vectors for bioplastics production in oil palm |
| url | http://psasir.upm.edu.my/id/eprint/13759/1/Construction%20of%20PHB%20and%20PHBV%20transformation%20vectors%20for%20bioplastics%20production%20in%20oil%20palm.pdf |
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