Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli.
The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-fr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Asian Network for Scientific Information
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13785/1/Controlled%20expression%20of%20cholera%20toxin%20B%20subunit%20from%20Vibro%20cholerae%20in%20Escherichia%20coli.pdf |
| _version_ | 1796968510698553344 |
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| author | Toosa, Haryanti Shamsudin , Mariana Nor Saiful Yazan, Latifah Yusoff, Khatijah Abdul Rahim, Raha |
| author_facet | Toosa, Haryanti Shamsudin , Mariana Nor Saiful Yazan, Latifah Yusoff, Khatijah Abdul Rahim, Raha |
| author_sort | Toosa, Haryanti |
| collection | UPM |
| description | The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-frame with the P(BAD) promoter and it showed that the gene was 99% homologous to the published ctxB sequence. The CTB protein was successfully expressed in E. coli using the pBAD/His vector system. The expected protein of approximately 14 kDa was detected by SDS-PAGE and Western blot. The use of pBAD/His vector to express the cholera toxin gene in E. coli would facilitate future study of toxin gene products. |
| first_indexed | 2024-03-06T07:29:23Z |
| format | Article |
| id | upm.eprints-13785 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:29:23Z |
| publishDate | 2008 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | upm.eprints-137852016-01-20T03:50:55Z http://psasir.upm.edu.my/id/eprint/13785/ Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. Toosa, Haryanti Shamsudin , Mariana Nor Saiful Yazan, Latifah Yusoff, Khatijah Abdul Rahim, Raha The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-frame with the P(BAD) promoter and it showed that the gene was 99% homologous to the published ctxB sequence. The CTB protein was successfully expressed in E. coli using the pBAD/His vector system. The expected protein of approximately 14 kDa was detected by SDS-PAGE and Western blot. The use of pBAD/His vector to express the cholera toxin gene in E. coli would facilitate future study of toxin gene products. Asian Network for Scientific Information 2008-07-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13785/1/Controlled%20expression%20of%20cholera%20toxin%20B%20subunit%20from%20Vibro%20cholerae%20in%20Escherichia%20coli.pdf Toosa, Haryanti and Shamsudin , Mariana Nor and Saiful Yazan, Latifah and Yusoff, Khatijah and Abdul Rahim, Raha (2008) Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. Pakistan Journal of Biological Sciences, 11 (13). pp. 1718-1722. ISSN 1028-8880; ESSN: 1812-5735 English |
| spellingShingle | Toosa, Haryanti Shamsudin , Mariana Nor Saiful Yazan, Latifah Yusoff, Khatijah Abdul Rahim, Raha Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. |
| title | Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. |
| title_full | Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. |
| title_fullStr | Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. |
| title_full_unstemmed | Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. |
| title_short | Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli. |
| title_sort | controlled expression of cholera toxin b subunit from vibro cholerae in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/13785/1/Controlled%20expression%20of%20cholera%20toxin%20B%20subunit%20from%20Vibro%20cholerae%20in%20Escherichia%20coli.pdf |
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