Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells
2’,3’-Epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol are two bioactive compounds isolated from the leaves of Micromelum minutum. In this study, the induction of apoptosis by 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol on CEM-SS cells were investigated. The cytoto...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13858/1/13858.pdf |
| _version_ | 1825945316957880320 |
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| author | Tan, Kar Ban Mohammed Alitheen, Noorjahan Banu Yeap, Swee Keong Ali, Abdul Manaf Rahmani, Mawardi |
| author_facet | Tan, Kar Ban Mohammed Alitheen, Noorjahan Banu Yeap, Swee Keong Ali, Abdul Manaf Rahmani, Mawardi |
| author_sort | Tan, Kar Ban |
| collection | UPM |
| description | 2’,3’-Epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol are two bioactive compounds isolated from the leaves of Micromelum minutum. In this study, the induction of apoptosis by 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol on CEM-SS cells were investigated. The cytotoxicity of 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol was screened on CEM-SS by using MTT assay. The apoptotic effect of both compounds on CEM-SS was evaluated by using fluorescence and electron microcopies morphological observation and DNA laddering analysis. The inhibition effect of 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol at 50% of cell population (IC50) was found to be 4.6 µg/ml (13.5 µM) and 3 µg/ml (7.8 µM) on CEM-SS cells, respectively. From DNA fragmentation, Acridine orange and Propidium iodide staining and electron microscope analyses, the compounds were confirmed to have ability in promoting apoptosis. However, the % of apoptosis induced is low and the event is time-dependent. At high concentration of 10 µg/mL,2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol induced necrosis. Furthermore, 8-hydroxyisocapnolactone-2’,3’-diol also exhibited better cytotoxicity compared to 2’,3’-epoxyisocap-nolactone. The induction time for apoptosis by 8-hydroxyisocapnolactone-2’,3’-diol in CEM-SS is earlier than 2’,3’-epoxyisocapnolactone, which is 4 h and 12 h after treatment. Both 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol are potential as anticancer agent. |
| first_indexed | 2024-03-06T07:29:36Z |
| format | Article |
| id | upm.eprints-13858 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:29:36Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-138582016-05-03T11:00:58Z http://psasir.upm.edu.my/id/eprint/13858/ Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells Tan, Kar Ban Mohammed Alitheen, Noorjahan Banu Yeap, Swee Keong Ali, Abdul Manaf Rahmani, Mawardi 2’,3’-Epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol are two bioactive compounds isolated from the leaves of Micromelum minutum. In this study, the induction of apoptosis by 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol on CEM-SS cells were investigated. The cytotoxicity of 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol was screened on CEM-SS by using MTT assay. The apoptotic effect of both compounds on CEM-SS was evaluated by using fluorescence and electron microcopies morphological observation and DNA laddering analysis. The inhibition effect of 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol at 50% of cell population (IC50) was found to be 4.6 µg/ml (13.5 µM) and 3 µg/ml (7.8 µM) on CEM-SS cells, respectively. From DNA fragmentation, Acridine orange and Propidium iodide staining and electron microscope analyses, the compounds were confirmed to have ability in promoting apoptosis. However, the % of apoptosis induced is low and the event is time-dependent. At high concentration of 10 µg/mL,2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol induced necrosis. Furthermore, 8-hydroxyisocapnolactone-2’,3’-diol also exhibited better cytotoxicity compared to 2’,3’-epoxyisocap-nolactone. The induction time for apoptosis by 8-hydroxyisocapnolactone-2’,3’-diol in CEM-SS is earlier than 2’,3’-epoxyisocapnolactone, which is 4 h and 12 h after treatment. Both 2’,3’-epoxyisocapnolactone and 8-hydroxyisocapnolactone-2’,3’-diol are potential as anticancer agent. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13858/1/13858.pdf Tan, Kar Ban and Mohammed Alitheen, Noorjahan Banu and Yeap, Swee Keong and Ali, Abdul Manaf and Rahmani, Mawardi (2009) Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells. African Journal of Biotechnology, 8 (18). art. no. F48A1C29321. pp. 4632-4641. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/F48A1C29321 10.5897/AJB09.261 |
| spellingShingle | Tan, Kar Ban Mohammed Alitheen, Noorjahan Banu Yeap, Swee Keong Ali, Abdul Manaf Rahmani, Mawardi Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells |
| title | Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells |
| title_full | Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells |
| title_fullStr | Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells |
| title_full_unstemmed | Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells |
| title_short | Cytotoxic effect of 2’, 3’-epoxy isocapnolactone and 8-hydroxyisocapnolactone-2’3’-diol isolated from Micromelum minutum (G.Forst.) Wight and Arn. in human T-lymphocyte leukemia CEM-SS cells |
| title_sort | cytotoxic effect of 2 3 epoxy isocapnolactone and 8 hydroxyisocapnolactone 2 3 diol isolated from micromelum minutum g forst wight and arn in human t lymphocyte leukemia cem ss cells |
| url | http://psasir.upm.edu.my/id/eprint/13858/1/13858.pdf |
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