Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution
Defoliation is a common phenomenon which usually hinders the productivity of plantlets of Azadirachta excelsa through micropropagation. Thus, a study to develop a procedure to overcome defoliation of in vitro shootlets of A. excelsa after undergoing rooting stage was conducted. Shoots exhibiting def...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Forestry Department Peninsular Malaysia
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13882/1/Defoliation%20of%20in%20vitro%20shootlets%20of%20Azadirachta%20excelsa.pdf |
| _version_ | 1825945321618800640 |
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| author | Ab. Shukor, Nor Aini Jainol, Judy E. Mohd Yusof, Aziah Abdul Kadir, Mihdzar |
| author_facet | Ab. Shukor, Nor Aini Jainol, Judy E. Mohd Yusof, Aziah Abdul Kadir, Mihdzar |
| author_sort | Ab. Shukor, Nor Aini |
| collection | UPM |
| description | Defoliation is a common phenomenon which usually hinders the productivity of plantlets of Azadirachta excelsa through micropropagation. Thus, a study to develop a procedure to overcome defoliation of in vitro shootlets of A. excelsa after undergoing rooting stage was conducted. Shoots exhibiting defoliation were transferred on to MS medium supplemented either with only 0.24% gelrite, mixture of 0.1 mgl-1 BAP, 1.0 mgl-1 NAA and 0.75% difco-bacto agar, mixture of 0.24% gelrite and 0.2% activated charcoal and mixture of 0.24% gelrite, 2.0 mgl-1 BAP and 10.0 mgl-1 AgNO3. The results indicated that the different treatment produced significant effects at P<0.05 on the number of leaves, shoot length, root length and number of axillary shoots after 30 days of culture incubation. The MS medium supplemented only with 0.24% gelrite was the most effective treatment producing the highest number of leaves (9.8) and the longest shoot and root lengths of 17.2 mm and 43.5 mm respectively. The addition of NAA, BAP and AgNO3 stimulated callus formation and growth of axillary shoot. |
| first_indexed | 2024-03-06T07:29:40Z |
| format | Article |
| id | upm.eprints-13882 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:29:40Z |
| publishDate | 2008 |
| publisher | Forestry Department Peninsular Malaysia |
| record_format | dspace |
| spelling | upm.eprints-138822015-12-02T07:07:26Z http://psasir.upm.edu.my/id/eprint/13882/ Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution Ab. Shukor, Nor Aini Jainol, Judy E. Mohd Yusof, Aziah Abdul Kadir, Mihdzar Defoliation is a common phenomenon which usually hinders the productivity of plantlets of Azadirachta excelsa through micropropagation. Thus, a study to develop a procedure to overcome defoliation of in vitro shootlets of A. excelsa after undergoing rooting stage was conducted. Shoots exhibiting defoliation were transferred on to MS medium supplemented either with only 0.24% gelrite, mixture of 0.1 mgl-1 BAP, 1.0 mgl-1 NAA and 0.75% difco-bacto agar, mixture of 0.24% gelrite and 0.2% activated charcoal and mixture of 0.24% gelrite, 2.0 mgl-1 BAP and 10.0 mgl-1 AgNO3. The results indicated that the different treatment produced significant effects at P<0.05 on the number of leaves, shoot length, root length and number of axillary shoots after 30 days of culture incubation. The MS medium supplemented only with 0.24% gelrite was the most effective treatment producing the highest number of leaves (9.8) and the longest shoot and root lengths of 17.2 mm and 43.5 mm respectively. The addition of NAA, BAP and AgNO3 stimulated callus formation and growth of axillary shoot. Forestry Department Peninsular Malaysia 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13882/1/Defoliation%20of%20in%20vitro%20shootlets%20of%20Azadirachta%20excelsa.pdf Ab. Shukor, Nor Aini and Jainol, Judy E. and Mohd Yusof, Aziah and Abdul Kadir, Mihdzar (2008) Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution. The Malaysian Forester, 71 (1). pp. 37-44. ISSN 0302-2935 http://www.cabi.org/cabdirect/FullTextPDF/2010/20103045586.pdf |
| spellingShingle | Ab. Shukor, Nor Aini Jainol, Judy E. Mohd Yusof, Aziah Abdul Kadir, Mihdzar Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution |
| title | Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution |
| title_full | Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution |
| title_fullStr | Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution |
| title_full_unstemmed | Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution |
| title_short | Defoliation of in vitro shootlets of Azadirachta excelsa (Jack) M. Jacobs: a possible solution |
| title_sort | defoliation of in vitro shootlets of azadirachta excelsa jack m jacobs a possible solution |
| url | http://psasir.upm.edu.my/id/eprint/13882/1/Defoliation%20of%20in%20vitro%20shootlets%20of%20Azadirachta%20excelsa.pdf |
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