Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens.
Background: Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). Study on the type of CAV isolates present and their genetic diversity, transmission to their progeny and level of protection afforded in the breeder farms is lacking in Malaysia. Hence, the present s...
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| Format: | Article |
| Language: | English English |
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BioMed Central
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13924/1/Detection%20and%20characterization%20of%20chicken%20anaemia%20virus%20from%20commercial%20broiler%20breeder%20chickens.pdf |
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| author | Negasi, Zerihun Hailemariam Omar, Abdul Rahman Bejo, Mohd Hair Tan, Ching Giap |
| author_facet | Negasi, Zerihun Hailemariam Omar, Abdul Rahman Bejo, Mohd Hair Tan, Ching Giap |
| author_sort | Negasi, Zerihun Hailemariam |
| collection | UPM |
| description | Background: Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia
(CIA). Study on the type of CAV isolates present and their genetic diversity, transmission to their
progeny and level of protection afforded in the breeder farms is lacking in Malaysia. Hence, the
present study was aimed to detect CAV from commercial broiler breeder farms and characterize
CAV positive samples based on sequence and phylogenetic analysis of partial VP1 gene.
Results: A total of 12 CAV isolates from different commercial broiler breeder farms were isolated
and characterized. Detection of CAV positive embryos by the PCR assay in the range of 40 to 100%
for different farms indicated high level of occurrence of vertical transmission of viral DNA to the
progeny. CAV antigen was detected in the thymus and in the bone marrow but not in spleen, liver,
duodenum, ovary and oviduct by indirect immunoperoxidase staining. The 12 CAV isolates were
characterized based on partial sequences of VP1 gene. Six isolates (MF1A, MF3C, M3B5, NF4A,
P12B and P24A) were found to have maximum homology with previously characterized Malaysian
isolate SMSC-1, four isolates (M1B1, NF3A, PYT4 and PPW4) with isolate BL-5 and the remaining
two (NF1D and NF2C) have maximum homology both with isolates 3-1 and BL-5. Meanwhile,
seven of the isolates with amino acid profile of 75-I, 97-L, 139-Q and 144-Q were clustered
together in cluster I together with other isolates from different geographical places. The remaining
five isolates with amino acid profile of 75-V, 97-M, 139-K and 144-E were grouped under cluster II.
All the CAV isolates demonstrated omega values (Ka/Ks) of less than one (the values ranging from
0.07 to 0.5) suggesting the occurrence of purifying (negative) selection in all the studied isolates.
Conclusion: The present study showed that CAV is widespread in the studied commercial broiler
breeder farms. The result also indicated the occurrence of genetic variability in local CAV isolates
that can be divided at least into two groups based on characteristic amino acid substitutions at
positions 75, 97, 139 and 144 of the VP1 protein. |
| first_indexed | 2024-03-06T07:29:48Z |
| format | Article |
| id | upm.eprints-13924 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:29:48Z |
| publishDate | 2008 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | upm.eprints-139242015-12-22T04:31:28Z http://psasir.upm.edu.my/id/eprint/13924/ Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. Negasi, Zerihun Hailemariam Omar, Abdul Rahman Bejo, Mohd Hair Tan, Ching Giap Background: Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). Study on the type of CAV isolates present and their genetic diversity, transmission to their progeny and level of protection afforded in the breeder farms is lacking in Malaysia. Hence, the present study was aimed to detect CAV from commercial broiler breeder farms and characterize CAV positive samples based on sequence and phylogenetic analysis of partial VP1 gene. Results: A total of 12 CAV isolates from different commercial broiler breeder farms were isolated and characterized. Detection of CAV positive embryos by the PCR assay in the range of 40 to 100% for different farms indicated high level of occurrence of vertical transmission of viral DNA to the progeny. CAV antigen was detected in the thymus and in the bone marrow but not in spleen, liver, duodenum, ovary and oviduct by indirect immunoperoxidase staining. The 12 CAV isolates were characterized based on partial sequences of VP1 gene. Six isolates (MF1A, MF3C, M3B5, NF4A, P12B and P24A) were found to have maximum homology with previously characterized Malaysian isolate SMSC-1, four isolates (M1B1, NF3A, PYT4 and PPW4) with isolate BL-5 and the remaining two (NF1D and NF2C) have maximum homology both with isolates 3-1 and BL-5. Meanwhile, seven of the isolates with amino acid profile of 75-I, 97-L, 139-Q and 144-Q were clustered together in cluster I together with other isolates from different geographical places. The remaining five isolates with amino acid profile of 75-V, 97-M, 139-K and 144-E were grouped under cluster II. All the CAV isolates demonstrated omega values (Ka/Ks) of less than one (the values ranging from 0.07 to 0.5) suggesting the occurrence of purifying (negative) selection in all the studied isolates. Conclusion: The present study showed that CAV is widespread in the studied commercial broiler breeder farms. The result also indicated the occurrence of genetic variability in local CAV isolates that can be divided at least into two groups based on characteristic amino acid substitutions at positions 75, 97, 139 and 144 of the VP1 protein. BioMed Central 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13924/1/Detection%20and%20characterization%20of%20chicken%20anaemia%20virus%20from%20commercial%20broiler%20breeder%20chickens.pdf Negasi, Zerihun Hailemariam and Omar, Abdul Rahman and Bejo, Mohd Hair and Tan, Ching Giap (2008) Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. Virology Journal, 5 (128). pp. 1-11. ISSN 1743-422X 10.1186/1743-422X-5-128 English |
| spellingShingle | Negasi, Zerihun Hailemariam Omar, Abdul Rahman Bejo, Mohd Hair Tan, Ching Giap Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. |
| title | Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. |
| title_full | Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. |
| title_fullStr | Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. |
| title_full_unstemmed | Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. |
| title_short | Detection and characterization of chicken anaemia virus from commercial broiler breeder chickens. |
| title_sort | detection and characterization of chicken anaemia virus from commercial broiler breeder chickens |
| url | http://psasir.upm.edu.my/id/eprint/13924/1/Detection%20and%20characterization%20of%20chicken%20anaemia%20virus%20from%20commercial%20broiler%20breeder%20chickens.pdf |
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