Methods for precise molecular detection of probiotic microflora : using adjusted molecular biology protocols, primer sets and PCR assays.
Lactobacillus sp. is probiotic bacteria for which many detection methods were envisaged. However, culture-based methods failed to achieve specific detection of this bacterium due to its presence in mixed bacterial complex communities. The PCR assay was optimized to detect and quantify Lactobacillus...
Main Authors: | Abu Bakar, Fatimah, Abdulamir, Ahmed Sahib, Nordin, Noordiana, Yoke, T. S. |
---|---|
Format: | Article |
Language: | English English |
Published: |
Asian Network for Scientific Information
2010
|
Online Access: | http://psasir.upm.edu.my/id/eprint/13937/1/Methods%20for%20precise%20molecular%20detection%20of%20probiotic%20microflora.pdf |
Similar Items
-
Detection and quantification of probiotic bacteria using optimized DNA extraction, traditional and real-time PCR methods in complex microbial communities
by: Abdulamir, Ahmed Sahib, et al.
Published: (2010) -
Gne-primer : a PCR primer design program
by: Kok, Wui Lim.
Published: (2008) -
Gene-primer a PCR primer design program
by: Kok, Wui Lim.
Published: (2008) -
Development of a diagnostic real time pcr assay for molecular detection and characterization of mycoplasma gallisepticum
by: Yasmin, Farhana
Published: (2013) -
Development of a diagnostic real time pcr assay for molecular detection and characterization of mycoplasma gallisepticum
by: Yasmin, Farhana
Published: (2013)