Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
: The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and se...
| Главные авторы: | , , , |
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| Формат: | Статья |
| Язык: | English English English |
| Опубликовано: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online-ссылка: | http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf |
| _version_ | 1825945350389628928 |
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| author | Cheah, Yoke Kqueen Chong, Yee Tyan Khoo, Siew Ping Radu, Son |
| author_facet | Cheah, Yoke Kqueen Chong, Yee Tyan Khoo, Siew Ping Radu, Son |
| author_sort | Cheah, Yoke Kqueen |
| collection | UPM |
| description | : The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and
time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for
GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and secondly
to obtain purified nucleic acids using CTAB DNA extraction method for conducting a GM specific analysis
on various types of food samples. The multiplex PCR was optimized to improve PCR performance and to
minimize failure. Out of 60 samples, 42 (70.0%) were found containing Cry1Ab or EPSPS genes, consisting of
11.9% of Roundup Ready Soya positive samples and 88.1% of Bt 176 Maize positive samples. Besides, 71.7%
samples yielded DNA concentration above 50 ng/µl; 66.7% samples were in the DNA purity range of 1.6 to
2.0 and 85.0% of the samples were amplifiable for the endogenous gene screening. The CTAB DNA extraction
method is effective for the DNA extraction from animal feeds, raw materials and processed foods. |
| first_indexed | 2024-03-06T07:30:07Z |
| format | Article |
| id | upm.eprints-14031 |
| institution | Universiti Putra Malaysia |
| language | English English English |
| last_indexed | 2024-03-06T07:30:07Z |
| publishDate | 2011 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-140312015-10-06T01:49:38Z http://psasir.upm.edu.my/id/eprint/14031/ Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples Cheah, Yoke Kqueen Chong, Yee Tyan Khoo, Siew Ping Radu, Son : The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and secondly to obtain purified nucleic acids using CTAB DNA extraction method for conducting a GM specific analysis on various types of food samples. The multiplex PCR was optimized to improve PCR performance and to minimize failure. Out of 60 samples, 42 (70.0%) were found containing Cry1Ab or EPSPS genes, consisting of 11.9% of Roundup Ready Soya positive samples and 88.1% of Bt 176 Maize positive samples. Besides, 71.7% samples yielded DNA concentration above 50 ng/µl; 66.7% samples were in the DNA purity range of 1.6 to 2.0 and 85.0% of the samples were amplifiable for the endogenous gene screening. The CTAB DNA extraction method is effective for the DNA extraction from animal feeds, raw materials and processed foods. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf Cheah, Yoke Kqueen and Chong, Yee Tyan and Khoo, Siew Ping and Radu, Son (2011) Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples. International Food Research Journal, 18 (2). pp. 515-522. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html English |
| spellingShingle | Cheah, Yoke Kqueen Chong, Yee Tyan Khoo, Siew Ping Radu, Son Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples |
| title | Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples |
| title_full | Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples |
| title_fullStr | Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples |
| title_full_unstemmed | Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples |
| title_short | Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples |
| title_sort | development of multiplex pcr for genetically modified organism gmo detection targeting epsps and cry1ab genes in soy and maize samples |
| url | http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf |
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