Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles
In the present study, distinctive RAPD fingerprints were generated for 12 Lactobacillus-probiotic strains from 5 Lactobacillus species (L. brevis, L. reuteri, L. gallinarium, L. salivarius and L. panis) after optimization of the RAPD parameters such as MgCl2, Taq polymerase, primer concentration and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14071/1/14071.pdf |
| _version_ | 1796968569700876288 |
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| author | Abdul Manan, Norlida Sieo, Chin Chin Abdullah, Norhani Ho, Yin Wan |
| author_facet | Abdul Manan, Norlida Sieo, Chin Chin Abdullah, Norhani Ho, Yin Wan |
| author_sort | Abdul Manan, Norlida |
| collection | UPM |
| description | In the present study, distinctive RAPD fingerprints were generated for 12 Lactobacillus-probiotic strains from 5 Lactobacillus species (L. brevis, L. reuteri, L. gallinarium, L. salivarius and L. panis) after optimization of the RAPD parameters such as MgCl2, Taq polymerase, primer concentration and type of primer. The strains were differentiated under the same PCR protocol but different concentration of primer OPM-05 (50 pmole to differentiate the 5 L. brevis strains and 75 pmole to differentiate 2 strains of L. gallinarium, 3 strains of L. reuteri, a strain of L. panis and L. salivarius). The RAPD fingerprints generated could be differentiated by visual comparison of the profiles, without being analysed by relevant software. This allows specific, rapid, immediate and convenient identification of the Lactobacillus strains. |
| first_indexed | 2024-03-06T07:30:14Z |
| format | Article |
| id | upm.eprints-14071 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:30:14Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-140712017-11-09T05:26:21Z http://psasir.upm.edu.my/id/eprint/14071/ Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles Abdul Manan, Norlida Sieo, Chin Chin Abdullah, Norhani Ho, Yin Wan In the present study, distinctive RAPD fingerprints were generated for 12 Lactobacillus-probiotic strains from 5 Lactobacillus species (L. brevis, L. reuteri, L. gallinarium, L. salivarius and L. panis) after optimization of the RAPD parameters such as MgCl2, Taq polymerase, primer concentration and type of primer. The strains were differentiated under the same PCR protocol but different concentration of primer OPM-05 (50 pmole to differentiate the 5 L. brevis strains and 75 pmole to differentiate 2 strains of L. gallinarium, 3 strains of L. reuteri, a strain of L. panis and L. salivarius). The RAPD fingerprints generated could be differentiated by visual comparison of the profiles, without being analysed by relevant software. This allows specific, rapid, immediate and convenient identification of the Lactobacillus strains. Academic Journals 2009-08-18 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14071/1/14071.pdf Abdul Manan, Norlida and Sieo, Chin Chin and Abdullah, Norhani and Ho, Yin Wan (2009) Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles. African Journal of Biotechnology, 8 (16). art. no. ED0B1398389. pp. 3964-3969. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/ED0B1398389 |
| spellingShingle | Abdul Manan, Norlida Sieo, Chin Chin Abdullah, Norhani Ho, Yin Wan Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles |
| title | Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles |
| title_full | Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles |
| title_fullStr | Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles |
| title_full_unstemmed | Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles |
| title_short | Differentiation of Lactobacillus-probiotic strains by visual comparison of random amplified polymorphic DNA (RAPD) profiles |
| title_sort | differentiation of lactobacillus probiotic strains by visual comparison of random amplified polymorphic dna rapd profiles |
| url | http://psasir.upm.edu.my/id/eprint/14071/1/14071.pdf |
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