Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells
Hepatitis B is a major public health problem worldwide which may lead to chronic liver diseases, cirrhosis and hepatocellular carcinoma. An interaction between hepatitis B virus (HBV) envelope protein, particularly the PreS1 region, and a specific cell surface receptor is believed to be the initial...
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| Format: | Article |
| Language: | English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14097/1/Display%20of%20hepatitis%20B%20virus%20PreS1%20peptide%20on%20bacteriophage%20T7%20and%20its%20potential%20in%20gene%20delivery%20into%20HepG2%20cells.pdf |
| _version_ | 1825945363094175744 |
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| author | Tang, Kie Hie Mohd Yusoff, Khatijah Tan, Wen Siang |
| author_facet | Tang, Kie Hie Mohd Yusoff, Khatijah Tan, Wen Siang |
| author_sort | Tang, Kie Hie |
| collection | UPM |
| description | Hepatitis B is a major public health problem worldwide which may lead to chronic liver diseases, cirrhosis and hepatocellular carcinoma. An interaction between hepatitis B virus (HBV) envelope protein, particularly the PreS1 region, and a specific cell surface receptor is believed to be the initial step of HBV infection through attachment to hepatocytes. In order to develop a gene delivery system, bacteriophage T7 was modified genetically to display polypeptides of the PreS1 region. A recombinant T7 phage displaying amino acids 60-108 of the PreS1 region (PreS1(60-108)) was demonstrated to be most effective in transfecting HepG2 cells in a dose- and time-dependant manner. The phage genome was recovered from the cell lysate and confirmed by PCR whereas the infectious form of the internalized phage was measured by a plaque-forming assay. The internalized phage exhibited the appearance of green fluorescent dots when examined by immunofluorescence microscopy. Surface modification, particularly by displaying the PreS1(60-108) enhanced phage uptake, resulting in more efficient in vitro gene transfer. The ability of the recombinant phage to transfect HepG2 cells demonstrates the potential of the phage display system as a gene therapy for liver cancer. |
| first_indexed | 2024-03-06T07:30:19Z |
| format | Article |
| id | upm.eprints-14097 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:30:19Z |
| publishDate | 2009 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-140972016-11-30T09:41:10Z http://psasir.upm.edu.my/id/eprint/14097/ Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells Tang, Kie Hie Mohd Yusoff, Khatijah Tan, Wen Siang Hepatitis B is a major public health problem worldwide which may lead to chronic liver diseases, cirrhosis and hepatocellular carcinoma. An interaction between hepatitis B virus (HBV) envelope protein, particularly the PreS1 region, and a specific cell surface receptor is believed to be the initial step of HBV infection through attachment to hepatocytes. In order to develop a gene delivery system, bacteriophage T7 was modified genetically to display polypeptides of the PreS1 region. A recombinant T7 phage displaying amino acids 60-108 of the PreS1 region (PreS1(60-108)) was demonstrated to be most effective in transfecting HepG2 cells in a dose- and time-dependant manner. The phage genome was recovered from the cell lysate and confirmed by PCR whereas the infectious form of the internalized phage was measured by a plaque-forming assay. The internalized phage exhibited the appearance of green fluorescent dots when examined by immunofluorescence microscopy. Surface modification, particularly by displaying the PreS1(60-108) enhanced phage uptake, resulting in more efficient in vitro gene transfer. The ability of the recombinant phage to transfect HepG2 cells demonstrates the potential of the phage display system as a gene therapy for liver cancer. Elsevier 2009-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14097/1/Display%20of%20hepatitis%20B%20virus%20PreS1%20peptide%20on%20bacteriophage%20T7%20and%20its%20potential%20in%20gene%20delivery%20into%20HepG2%20cells.pdf Tang, Kie Hie and Mohd Yusoff, Khatijah and Tan, Wen Siang (2009) Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells. Journal of Virological Methods, 159 (2). pp. 194-199. ISSN 0166-0934; ESSN: 1879-0984 10.1016/j.jviromet.2009.03.015 |
| spellingShingle | Tang, Kie Hie Mohd Yusoff, Khatijah Tan, Wen Siang Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells |
| title | Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells |
| title_full | Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells |
| title_fullStr | Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells |
| title_full_unstemmed | Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells |
| title_short | Display of hepatitis B virus PreS1 peptide on bacteriophage T7 and its potential in gene delivery into HepG2 cells |
| title_sort | display of hepatitis b virus pres1 peptide on bacteriophage t7 and its potential in gene delivery into hepg2 cells |
| url | http://psasir.upm.edu.my/id/eprint/14097/1/Display%20of%20hepatitis%20B%20virus%20PreS1%20peptide%20on%20bacteriophage%20T7%20and%20its%20potential%20in%20gene%20delivery%20into%20HepG2%20cells.pdf |
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