Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus
The anther-derived embryoid cultures of Brassica napus is stably embryogenic and has an extracellular matrix (ECM) layer covering the surface of the developing embryoids. In this study, the distribution of arabinogalactan protein (AGP) epitopes in the ECM layer and the embryogenic tissue of winter o...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14106/1/20%20Pg%20303-313.pdf |
| _version_ | 1796968577103822848 |
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| author | Namasivayam, Parameswari Skepper, Jeremy Neil Hanke, David E. |
| author_facet | Namasivayam, Parameswari Skepper, Jeremy Neil Hanke, David E. |
| author_sort | Namasivayam, Parameswari |
| collection | UPM |
| description | The anther-derived embryoid cultures of Brassica napus is stably embryogenic and has an extracellular matrix (ECM) layer covering the surface of the developing embryoids. In this study, the distribution of arabinogalactan protein (AGP) epitopes in the ECM layer and the embryogenic tissue of winter oilseed rape were investigated by immuno-labelling with anti-AGP monoclonal antibodies (mAb JIM4, JIM8, and JIM 13). There was no labelling by the JIM4 and JIM8 mAbs in the ECM layer, unlike what was reported in other plant species. JIM 13 epitope is developmentally regulated because it was only present in the ECM layer of the mature embryogenic tissue. These observations indicate a possible variability in the AGP epitopes present in the ECM layer among the different plant species. JIM8 and JIM 13 epitopes were found in some epidermal cells of embryogenic tissue, but not in the non-embryogenic tissue, implying that AGPs might have a specific role in embryogenic competency or determining the cell fate of the B. napus embryogenic cells |
| first_indexed | 2024-03-06T07:30:21Z |
| format | Article |
| id | upm.eprints-14106 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:30:21Z |
| publishDate | 2010 |
| publisher | Universiti Putra Malaysia Press |
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| spelling | upm.eprints-141062016-12-07T03:40:00Z http://psasir.upm.edu.my/id/eprint/14106/ Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus Namasivayam, Parameswari Skepper, Jeremy Neil Hanke, David E. The anther-derived embryoid cultures of Brassica napus is stably embryogenic and has an extracellular matrix (ECM) layer covering the surface of the developing embryoids. In this study, the distribution of arabinogalactan protein (AGP) epitopes in the ECM layer and the embryogenic tissue of winter oilseed rape were investigated by immuno-labelling with anti-AGP monoclonal antibodies (mAb JIM4, JIM8, and JIM 13). There was no labelling by the JIM4 and JIM8 mAbs in the ECM layer, unlike what was reported in other plant species. JIM 13 epitope is developmentally regulated because it was only present in the ECM layer of the mature embryogenic tissue. These observations indicate a possible variability in the AGP epitopes present in the ECM layer among the different plant species. JIM8 and JIM 13 epitopes were found in some epidermal cells of embryogenic tissue, but not in the non-embryogenic tissue, implying that AGPs might have a specific role in embryogenic competency or determining the cell fate of the B. napus embryogenic cells Universiti Putra Malaysia Press 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14106/1/20%20Pg%20303-313.pdf Namasivayam, Parameswari and Skepper, Jeremy Neil and Hanke, David E. (2010) Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus. Pertanika Journal of Tropical Agricultural Science, 33 (2). pp. 303-313. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2033%20(2)%20Aug.%202010/20%20Pg%20303-313.pdf |
| spellingShingle | Namasivayam, Parameswari Skepper, Jeremy Neil Hanke, David E. Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus |
| title | Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus |
| title_full | Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus |
| title_fullStr | Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus |
| title_full_unstemmed | Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus |
| title_short | Distribution of arabinogalactan protein (AGP) epitopes on the anther derived embryoid cultures of Brassica napus |
| title_sort | distribution of arabinogalactan protein agp epitopes on the anther derived embryoid cultures of brassica napus |
| url | http://psasir.upm.edu.my/id/eprint/14106/1/20%20Pg%20303-313.pdf |
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