Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus
The distribution of lectin bindings in the testis of the smallest ruminant, lesser mouse deer (Tragulus javanicus), was studied using 12 biotinylated lectins specific for d-galactose (peanut agglutinin PNA, Ricinus communis agglutinin RCA I), N-acetyl-d-galactosamine (Dolichos biflorus agglutinin DB...
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| Formaat: | Artikel |
| Taal: | English |
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Blackwell Verlag GmbH
2009
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| Online toegang: | http://psasir.upm.edu.my/id/eprint/14112/1/Distribution%20of%20lectin-bindings%20in%20the%20testis%20of%20the%20lesser%20mouse%20deer%2C%20Tragulus%20javanicus.pdf |
| _version_ | 1825945366025994240 |
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| author | Agungpriyono, Srihadi Kurohmaru, Masamichi Kimura, Junpei Haron, Abd Wahid Sasaki, Motoki Kitamura, Nobuo Yamada, Junzo Fukuta, Katsuhiro Abu Bakar @ Zakaria, Md Zuki |
| author_facet | Agungpriyono, Srihadi Kurohmaru, Masamichi Kimura, Junpei Haron, Abd Wahid Sasaki, Motoki Kitamura, Nobuo Yamada, Junzo Fukuta, Katsuhiro Abu Bakar @ Zakaria, Md Zuki |
| author_sort | Agungpriyono, Srihadi |
| collection | UPM |
| description | The distribution of lectin bindings in the testis of the smallest ruminant, lesser mouse deer (Tragulus javanicus), was studied using 12 biotinylated lectins specific for d-galactose (peanut agglutinin PNA, Ricinus communis agglutinin RCA I), N-acetyl-d-galactosamine (Dolichos biflorus agglutinin DBA, Vicia villosa agglutinin VVA, Soybean agglutinin SBA), N-acetyl-d-glucosamine and sialic acid (wheat germ agglutinin WGA, s-WGA), d-mannose and d-glucose (Lens culinaris agglutinin LCA, Pisum sativum agglutinin PSA, Concanavalin A Con A), l-fucose (Ulex europaeus agglutinin UEA I), and oligosaccharide (Phaseolus vulgaris agglutinin PHA-E) sugar residues. In Golgi-, cap-, and acrosome-phase spermatids, lectin-bindings were found in the acrosome (PNA, RCA I, VVA, SBA, WGA and s-WGA), and in the cytoplasm (PNA, RCA I, VVA, SBA, WGA, LCA, PSA, Con A and PHA-E). s-WGA binding was confined to the spermatid acrosome, but other lectins were also observed in spermatocytes. In spermatogonia, VVA, WGA, Con A, and PHA-E bindings were observed. Sertoli cells were intensely stained with DBA and Con A, and weakly with PHA-E. In interstitial Leydig cells, RCA I, DBA, VVA, Con A, PSA, LCA, WGA and PHA-E were positive. UEA I was negative in all cell types including spermatogenic cells. Unusual distribution of lectin-bindings noted in the testis of lesser mouse deer included the limited distribution of s-WGA only in the spermatid acrosome, the distribution of DBA in Sertoli cells, Leydig cells and lamina propria, and the absence of UEA I in all type cells. The present results were discussed in comparison with those of other animals and their possible functional implications. |
| first_indexed | 2024-03-06T07:30:22Z |
| format | Article |
| id | upm.eprints-14112 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:30:22Z |
| publishDate | 2009 |
| publisher | Blackwell Verlag GmbH |
| record_format | dspace |
| spelling | upm.eprints-141122016-11-18T02:09:38Z http://psasir.upm.edu.my/id/eprint/14112/ Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus Agungpriyono, Srihadi Kurohmaru, Masamichi Kimura, Junpei Haron, Abd Wahid Sasaki, Motoki Kitamura, Nobuo Yamada, Junzo Fukuta, Katsuhiro Abu Bakar @ Zakaria, Md Zuki The distribution of lectin bindings in the testis of the smallest ruminant, lesser mouse deer (Tragulus javanicus), was studied using 12 biotinylated lectins specific for d-galactose (peanut agglutinin PNA, Ricinus communis agglutinin RCA I), N-acetyl-d-galactosamine (Dolichos biflorus agglutinin DBA, Vicia villosa agglutinin VVA, Soybean agglutinin SBA), N-acetyl-d-glucosamine and sialic acid (wheat germ agglutinin WGA, s-WGA), d-mannose and d-glucose (Lens culinaris agglutinin LCA, Pisum sativum agglutinin PSA, Concanavalin A Con A), l-fucose (Ulex europaeus agglutinin UEA I), and oligosaccharide (Phaseolus vulgaris agglutinin PHA-E) sugar residues. In Golgi-, cap-, and acrosome-phase spermatids, lectin-bindings were found in the acrosome (PNA, RCA I, VVA, SBA, WGA and s-WGA), and in the cytoplasm (PNA, RCA I, VVA, SBA, WGA, LCA, PSA, Con A and PHA-E). s-WGA binding was confined to the spermatid acrosome, but other lectins were also observed in spermatocytes. In spermatogonia, VVA, WGA, Con A, and PHA-E bindings were observed. Sertoli cells were intensely stained with DBA and Con A, and weakly with PHA-E. In interstitial Leydig cells, RCA I, DBA, VVA, Con A, PSA, LCA, WGA and PHA-E were positive. UEA I was negative in all cell types including spermatogenic cells. Unusual distribution of lectin-bindings noted in the testis of lesser mouse deer included the limited distribution of s-WGA only in the spermatid acrosome, the distribution of DBA in Sertoli cells, Leydig cells and lamina propria, and the absence of UEA I in all type cells. The present results were discussed in comparison with those of other animals and their possible functional implications. Blackwell Verlag GmbH 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14112/1/Distribution%20of%20lectin-bindings%20in%20the%20testis%20of%20the%20lesser%20mouse%20deer%2C%20Tragulus%20javanicus.pdf Agungpriyono, Srihadi and Kurohmaru, Masamichi and Kimura, Junpei and Haron, Abd Wahid and Sasaki, Motoki and Kitamura, Nobuo and Yamada, Junzo and Fukuta, Katsuhiro and Abu Bakar @ Zakaria, Md Zuki (2009) Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus. Anatomia, Histologia, Embryologia, 38 (3). pp. 208-213. ISSN 0340-2096; ESSN: 1439-0264 http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1439-0264.2008.00923.x/abstract 10.1111/j.1439-0264.2008.00923.x |
| spellingShingle | Agungpriyono, Srihadi Kurohmaru, Masamichi Kimura, Junpei Haron, Abd Wahid Sasaki, Motoki Kitamura, Nobuo Yamada, Junzo Fukuta, Katsuhiro Abu Bakar @ Zakaria, Md Zuki Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus |
| title | Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus |
| title_full | Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus |
| title_fullStr | Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus |
| title_full_unstemmed | Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus |
| title_short | Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus |
| title_sort | distribution of lectin bindings in the testis of the lesser mouse deer tragulus javanicus |
| url | http://psasir.upm.edu.my/id/eprint/14112/1/Distribution%20of%20lectin-bindings%20in%20the%20testis%20of%20the%20lesser%20mouse%20deer%2C%20Tragulus%20javanicus.pdf |
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