DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR)
Forty three (n=43) genomic DNA of Escherichia coli (11 isolates from eggs and 32 isolates from imported beef meats) were characterized by shiga toxin 1 (stx1), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) analyses. In the shiga to...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14122/1/14122.pdf |
| _version_ | 1796968580735041536 |
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| author | Abd Mutalib, Sahilah L. Y. Y., Audrey Ong, S. L. Wan Nazri, Wan Sakeenah Shahimi, Safiyyah Abdullah Sani, Norrakiah Abdullah, Aminah Ariffin, Ahmad Azuhairi |
| author_facet | Abd Mutalib, Sahilah L. Y. Y., Audrey Ong, S. L. Wan Nazri, Wan Sakeenah Shahimi, Safiyyah Abdullah Sani, Norrakiah Abdullah, Aminah Ariffin, Ahmad Azuhairi |
| author_sort | Abd Mutalib, Sahilah |
| collection | UPM |
| description | Forty three (n=43) genomic DNA of Escherichia coli (11 isolates from eggs and 32 isolates from imported beef meats) were characterized by shiga toxin 1 (stx1), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) analyses. In the shiga toxin 1 (stx1) gene detection with primer stx 1F (5'-TTCTTCGGTATCCTATTCCC-3') and stx 1R (5'- CTGTCACAGTAACAACCGT-3'), 9 E. coli of beef meats isolates were positive toward sxt1 gene. The results of the ERIC-PCR and RAPD-PCR were analyzed using GelCompar II software. ERIC-PCR with primer ERIC1 (5'-CACTTAGGGGTCCTCGAATGTA -3') and ERIC2 (5'-AAGTAAGTGACTGGGGTGAGCG-3') discriminated the E. coli into 6 clusters and 10 single isolates at 80% similarity. RAPD-PCR with primer Gen8 and Gen9, produced 10 clusters and 15 single isolates and 12 clusters and 14 single isolates of 80%, respectively. These results demonstrated that both ERIC-PCR and RAPD-PCR are useful and suitable tools for molecular typing of those isolates examined. |
| first_indexed | 2024-03-06T07:30:24Z |
| format | Article |
| id | upm.eprints-14122 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:30:24Z |
| publishDate | 2010 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-141222015-09-25T03:07:23Z http://psasir.upm.edu.my/id/eprint/14122/ DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) Abd Mutalib, Sahilah L. Y. Y., Audrey Ong, S. L. Wan Nazri, Wan Sakeenah Shahimi, Safiyyah Abdullah Sani, Norrakiah Abdullah, Aminah Ariffin, Ahmad Azuhairi Forty three (n=43) genomic DNA of Escherichia coli (11 isolates from eggs and 32 isolates from imported beef meats) were characterized by shiga toxin 1 (stx1), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) analyses. In the shiga toxin 1 (stx1) gene detection with primer stx 1F (5'-TTCTTCGGTATCCTATTCCC-3') and stx 1R (5'- CTGTCACAGTAACAACCGT-3'), 9 E. coli of beef meats isolates were positive toward sxt1 gene. The results of the ERIC-PCR and RAPD-PCR were analyzed using GelCompar II software. ERIC-PCR with primer ERIC1 (5'-CACTTAGGGGTCCTCGAATGTA -3') and ERIC2 (5'-AAGTAAGTGACTGGGGTGAGCG-3') discriminated the E. coli into 6 clusters and 10 single isolates at 80% similarity. RAPD-PCR with primer Gen8 and Gen9, produced 10 clusters and 15 single isolates and 12 clusters and 14 single isolates of 80%, respectively. These results demonstrated that both ERIC-PCR and RAPD-PCR are useful and suitable tools for molecular typing of those isolates examined. Faculty of Food Science and Technology, Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14122/1/14122.pdf Abd Mutalib, Sahilah and L. Y. Y., Audrey and Ong, S. L. and Wan Nazri, Wan Sakeenah and Shahimi, Safiyyah and Abdullah Sani, Norrakiah and Abdullah, Aminah and Ariffin, Ahmad Azuhairi (2010) DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR). International Food Research Journal, 17 (4). pp. 853-866. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/17%20%2804%29%202010/%284%29%20IFRJ-2010-063%20Dr%20Sahilah%5B1%5D.pdf |
| spellingShingle | Abd Mutalib, Sahilah L. Y. Y., Audrey Ong, S. L. Wan Nazri, Wan Sakeenah Shahimi, Safiyyah Abdullah Sani, Norrakiah Abdullah, Aminah Ariffin, Ahmad Azuhairi DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) |
| title | DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) |
| title_full | DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) |
| title_fullStr | DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) |
| title_full_unstemmed | DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) |
| title_short | DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR) |
| title_sort | dna profiling among egg and beef meat isolates of escherichia coli by enterobacterial repetitive intergenic consensus pcr eric pcr and random amplified polymorphic dna pcr rapd pcr |
| url | http://psasir.upm.edu.my/id/eprint/14122/1/14122.pdf |
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