Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells
The possibility of using flocculation technique for the separation of microalgae, Chaetoceros calcitrans, biomass from the culture broth was investigated. The flocculation experiments were conducted in 500 mL beaker using culture broth obtained from 10 L photobioreactor. The harvesting efficiency of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14226/1/14226.pdf |
| _version_ | 1796968601943539712 |
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| author | Tuan Harith, Zuharlida Md. Yusoff, Fatimah Mohamed, Mohd Shamzi Mohamed Din, Mohamed Shariff Ariff, Arbakariya |
| author_facet | Tuan Harith, Zuharlida Md. Yusoff, Fatimah Mohamed, Mohd Shamzi Mohamed Din, Mohamed Shariff Ariff, Arbakariya |
| author_sort | Tuan Harith, Zuharlida |
| collection | UPM |
| description | The possibility of using flocculation technique for the separation of microalgae, Chaetoceros calcitrans, biomass from the culture broth was investigated. The flocculation experiments were conducted in 500 mL beaker using culture broth obtained from 10 L photobioreactor. The harvesting efficiency of 90 and 60% was obtained in flocculation without flocculants conducted for 10 days at 27ºC (in light and dark) and 4ºC (dark), respectively. Harvesting efficiency higher than 90% with short settling time was achieved by adjusting the culture pH to 10.2 using either sodium hydroxide (NaOH) or potassium hydroxide (KOH). Improved cell viability (> 80%) and settling time with a slight improvement of flocculation efficiency was achieved by the addition of polyelectrolytes flocculant (Magnafloc® LT 27 and LT 25). However, the flocculants were only functioned when the pH of the microalgae culture was pre-adjusted to a certain value that promotes cells entrapment and surface charge neutralization prior to flocculation process. The flocculation efficiency and cell viability obtained in flocculation with Magnafloc® (LT 25 and LT 27) was comparable to that obtained in flocculation with chitosan. When chitosan and Magnafloc® (LT 25 and LT 27) were used as flocculants, the highest flocculation efficiency of C. calcitrans cells was observed at pH 8 and 10.2, respectively. Substantial increased in sedimentation rate was observed with increasing flocculants dosage though the flocculation efficiency and cell viability were not significantly varied. |
| first_indexed | 2024-03-06T07:30:45Z |
| format | Article |
| id | upm.eprints-14226 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:30:45Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-142262016-05-03T11:23:26Z http://psasir.upm.edu.my/id/eprint/14226/ Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells Tuan Harith, Zuharlida Md. Yusoff, Fatimah Mohamed, Mohd Shamzi Mohamed Din, Mohamed Shariff Ariff, Arbakariya The possibility of using flocculation technique for the separation of microalgae, Chaetoceros calcitrans, biomass from the culture broth was investigated. The flocculation experiments were conducted in 500 mL beaker using culture broth obtained from 10 L photobioreactor. The harvesting efficiency of 90 and 60% was obtained in flocculation without flocculants conducted for 10 days at 27ºC (in light and dark) and 4ºC (dark), respectively. Harvesting efficiency higher than 90% with short settling time was achieved by adjusting the culture pH to 10.2 using either sodium hydroxide (NaOH) or potassium hydroxide (KOH). Improved cell viability (> 80%) and settling time with a slight improvement of flocculation efficiency was achieved by the addition of polyelectrolytes flocculant (Magnafloc® LT 27 and LT 25). However, the flocculants were only functioned when the pH of the microalgae culture was pre-adjusted to a certain value that promotes cells entrapment and surface charge neutralization prior to flocculation process. The flocculation efficiency and cell viability obtained in flocculation with Magnafloc® (LT 25 and LT 27) was comparable to that obtained in flocculation with chitosan. When chitosan and Magnafloc® (LT 25 and LT 27) were used as flocculants, the highest flocculation efficiency of C. calcitrans cells was observed at pH 8 and 10.2, respectively. Substantial increased in sedimentation rate was observed with increasing flocculants dosage though the flocculation efficiency and cell viability were not significantly varied. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14226/1/14226.pdf Tuan Harith, Zuharlida and Md. Yusoff, Fatimah and Mohamed, Mohd Shamzi and Mohamed Din, Mohamed Shariff and Ariff, Arbakariya (2009) Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells. African Journal of Biotechnology, 8 (21). art. no. 687D9389547. pp. 5971-5978. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/687D9389547 |
| spellingShingle | Tuan Harith, Zuharlida Md. Yusoff, Fatimah Mohamed, Mohd Shamzi Mohamed Din, Mohamed Shariff Ariff, Arbakariya Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells |
| title | Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells |
| title_full | Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells |
| title_fullStr | Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells |
| title_full_unstemmed | Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells |
| title_short | Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells |
| title_sort | effect of different flocculants on the flocculation performance of microalgae chaetoceros calcitrans cells |
| url | http://psasir.upm.edu.my/id/eprint/14226/1/14226.pdf |
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