Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC)
Background: Pasteurella multocida B:2 causes bovine haemorrhagic septicaemia (HS), leading to rapid fatalities in cattle and buffaloes. An attenuated derivative of P. multocida B:2 GDH7, was previously constructed through mutation of the gdhA gene and proved to be an effective live attenuated vaccin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14336/1/14336.pdf |
| _version_ | 1796968624270868480 |
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| author | Mohd Kamal, Nur Iqmaliza Akmal Saad, Mohd Zamri Masarudin, Mas Jaffri Othman, Siti Sarah |
| author_facet | Mohd Kamal, Nur Iqmaliza Akmal Saad, Mohd Zamri Masarudin, Mas Jaffri Othman, Siti Sarah |
| author_sort | Mohd Kamal, Nur Iqmaliza Akmal |
| collection | UPM |
| description | Background: Pasteurella multocida B:2 causes bovine haemorrhagic septicaemia (HS), leading to rapid fatalities in cattle and buffaloes. An attenuated derivative of P. multocida B:2 GDH7, was previously constructed through mutation of the gdhA gene and proved to be an effective live attenuated vaccine for HS. Currently, only two potential live attenuated vaccine candidates for HS are being reported; P. multocida B:2 GDH7 and P. multocida B:2 JRMT12. This study primarily aims to investigate the potential of P. multocida B:2 GDH7 strain as a delivery vehicle for DNA vaccine for future multivalent applications.
Results: An investigation on the adherence, invasion and intracellular survival of bacterial strains within the bovine aortic endothelial cell line (BAEC) were carried out. The potential vaccine strain, P. multocida B:2 GDH7, was significantly better (p ≤ 0.05) at adhering to and invading BAEC compared to its parent strain and to P. multocida B:2 JRMT12 and survived intracellularly 7 h post treatment, with a steady decline over time. A dual reporter plasmid, pSRGM, which enabled tracking of bacterial movement from the extracellular environment into the intracellular compartment of the mammalian cells, was subsequently transformed into P. multocida B:2 GDH7. Intracellular trafficking of the vaccine strain, P. multocida B:2 GDH7 was subsequently visualized by tracking the reporter proteins via confocal laser scanning microscopy (CLSM).
Conclusions: The ability of P. multocida B:2 GDH7 to model bactofection represents a possibility for this vaccine strain to be used as a delivery vehicle for DNA vaccine for future multivalent protection in cattle and buffaloes. |
| first_indexed | 2024-03-06T07:31:04Z |
| format | Article |
| id | upm.eprints-14336 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:31:04Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | upm.eprints-143362019-04-08T08:31:39Z http://psasir.upm.edu.my/id/eprint/14336/ Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) Mohd Kamal, Nur Iqmaliza Akmal Saad, Mohd Zamri Masarudin, Mas Jaffri Othman, Siti Sarah Background: Pasteurella multocida B:2 causes bovine haemorrhagic septicaemia (HS), leading to rapid fatalities in cattle and buffaloes. An attenuated derivative of P. multocida B:2 GDH7, was previously constructed through mutation of the gdhA gene and proved to be an effective live attenuated vaccine for HS. Currently, only two potential live attenuated vaccine candidates for HS are being reported; P. multocida B:2 GDH7 and P. multocida B:2 JRMT12. This study primarily aims to investigate the potential of P. multocida B:2 GDH7 strain as a delivery vehicle for DNA vaccine for future multivalent applications. Results: An investigation on the adherence, invasion and intracellular survival of bacterial strains within the bovine aortic endothelial cell line (BAEC) were carried out. The potential vaccine strain, P. multocida B:2 GDH7, was significantly better (p ≤ 0.05) at adhering to and invading BAEC compared to its parent strain and to P. multocida B:2 JRMT12 and survived intracellularly 7 h post treatment, with a steady decline over time. A dual reporter plasmid, pSRGM, which enabled tracking of bacterial movement from the extracellular environment into the intracellular compartment of the mammalian cells, was subsequently transformed into P. multocida B:2 GDH7. Intracellular trafficking of the vaccine strain, P. multocida B:2 GDH7 was subsequently visualized by tracking the reporter proteins via confocal laser scanning microscopy (CLSM). Conclusions: The ability of P. multocida B:2 GDH7 to model bactofection represents a possibility for this vaccine strain to be used as a delivery vehicle for DNA vaccine for future multivalent protection in cattle and buffaloes. BioMed Central 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14336/1/14336.pdf Mohd Kamal, Nur Iqmaliza Akmal and Saad, Mohd Zamri and Masarudin, Mas Jaffri and Othman, Siti Sarah (2017) Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC). BMC Veterinary Research, 13. art. no. 186. pp. 1-8. ISSN 1746-6148 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1109-1 10.1186/s12917-017-1109-1 |
| spellingShingle | Mohd Kamal, Nur Iqmaliza Akmal Saad, Mohd Zamri Masarudin, Mas Jaffri Othman, Siti Sarah Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) |
| title | Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) |
| title_full | Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) |
| title_fullStr | Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) |
| title_full_unstemmed | Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) |
| title_short | Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC) |
| title_sort | interaction between pasteurella multocida b 2 and its derivatives with bovine aortic endothelial cell baec |
| url | http://psasir.upm.edu.my/id/eprint/14336/1/14336.pdf |
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