Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).

An investigation into the effects of ethanol concentration (0–100%, v/v), extraction time (20–120 min) and extraction temperature (25–65 °C) on the extraction of phenolic antioxidants from mengkudu (Morinda citrifolia) was performed using a single-factor experiment. Total phenolic content (TPC) and...

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Main Authors: Thoo, Yin Yin, Ho, Swee Kheng, Liang, Jia Yun, Ho, Chun Wai, Tan, Chin Ping
Format: Article
Language:English
English
Published: Elsevier 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14347/1/Effects%20of%20binary%20solvent%20extraction%20system.pdf
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author Thoo, Yin Yin
Ho, Swee Kheng
Liang, Jia Yun
Ho, Chun Wai
Tan, Chin Ping
author_facet Thoo, Yin Yin
Ho, Swee Kheng
Liang, Jia Yun
Ho, Chun Wai
Tan, Chin Ping
author_sort Thoo, Yin Yin
collection UPM
description An investigation into the effects of ethanol concentration (0–100%, v/v), extraction time (20–120 min) and extraction temperature (25–65 °C) on the extraction of phenolic antioxidants from mengkudu (Morinda citrifolia) was performed using a single-factor experiment. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used for determination of phenolic compounds. Antioxidant capacity was evaluated by measuring the scavenging effect on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals. Experimental results showed that extraction conditions had significant effect on extraction of phenolic compounds and antioxidant capacities. The optimised conditions were 40% ethanol for 80 min at 65 °C, with values of 919.95 mg GAE/100 g DW for TPC, 472.73 mg CE/100 g DW for TFC, 791.71 μmol TEAC/100 g DW for ABTS and 1928.5 μmol TEAC/100 g DW for DPPH. TPC was significantly correlated with DPPH under the effects of ethanol concentration (r = 0.932) and extraction time (r = −0.938).
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spelling upm.eprints-143472016-01-15T07:59:39Z http://psasir.upm.edu.my/id/eprint/14347/ Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Thoo, Yin Yin Ho, Swee Kheng Liang, Jia Yun Ho, Chun Wai Tan, Chin Ping An investigation into the effects of ethanol concentration (0–100%, v/v), extraction time (20–120 min) and extraction temperature (25–65 °C) on the extraction of phenolic antioxidants from mengkudu (Morinda citrifolia) was performed using a single-factor experiment. Total phenolic content (TPC) and total flavonoid content (TFC) assays were used for determination of phenolic compounds. Antioxidant capacity was evaluated by measuring the scavenging effect on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals. Experimental results showed that extraction conditions had significant effect on extraction of phenolic compounds and antioxidant capacities. The optimised conditions were 40% ethanol for 80 min at 65 °C, with values of 919.95 mg GAE/100 g DW for TPC, 472.73 mg CE/100 g DW for TFC, 791.71 μmol TEAC/100 g DW for ABTS and 1928.5 μmol TEAC/100 g DW for DPPH. TPC was significantly correlated with DPPH under the effects of ethanol concentration (r = 0.932) and extraction time (r = −0.938). Elsevier 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14347/1/Effects%20of%20binary%20solvent%20extraction%20system.pdf Thoo, Yin Yin and Ho, Swee Kheng and Liang, Jia Yun and Ho, Chun Wai and Tan, Chin Ping (2010) Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chemistry, 120 (1). pp. 290-295. ISSN 0308-8146 10.1016/j.foodchem.2009.09.064 English
spellingShingle Thoo, Yin Yin
Ho, Swee Kheng
Liang, Jia Yun
Ho, Chun Wai
Tan, Chin Ping
Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).
title Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).
title_full Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).
title_fullStr Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).
title_full_unstemmed Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).
title_short Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia).
title_sort effects of binary solvent extraction system extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu morinda citrifolia
url http://psasir.upm.edu.my/id/eprint/14347/1/Effects%20of%20binary%20solvent%20extraction%20system.pdf
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