Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.

The effect of mild heat treatment (below gelatinization temperature) towards the susceptibility of granular starch to enzymatic hydrolysis was investigated. Tapioca and sweet potato starches were subjected to enzymatic hydrolysis with a mixture of fungal α-amylase and glucoamylase at 35 °C for 24 h....

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Main Authors: Yussof, Nor Shariffa, Alias, Abd Karim, Ariffin, Fazilah, Sarker, Zaidul Islam
Format: Article
Language:English
English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/14535/1/Enzymatic%20hydrolysis%20of%20granular%20native%20and%20mildly%20heat.pdf
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author Yussof, Nor Shariffa
Alias, Abd Karim
Ariffin, Fazilah
Sarker, Zaidul Islam
author_facet Yussof, Nor Shariffa
Alias, Abd Karim
Ariffin, Fazilah
Sarker, Zaidul Islam
author_sort Yussof, Nor Shariffa
collection UPM
description The effect of mild heat treatment (below gelatinization temperature) towards the susceptibility of granular starch to enzymatic hydrolysis was investigated. Tapioca and sweet potato starches were subjected to enzymatic hydrolysis with a mixture of fungal α-amylase and glucoamylase at 35 °C for 24 h. Starches were hydrolyzed in native (granular) state and after heat treatment below gelatinization temperature (60 °C for 30 min). The dextrose equivalent (DE) value of heat-treated starch increased significantly compared to native starch, i.e., 36–50% and 27–34% for tapioca and sweet potato starch, respectively. Scanning electron microscopy examination showed that enzymatic erosion occurred mainly at the surface of starch granules. Hydrolyzed heat-treated starch exhibited rougher surface and porous granules compared to native starch. X-ray analysis suggested that enzymatic erosion preferentially occurred in amorphous areas of the granules. The amylose content, swelling power and solubility showed insignificant increase for both starches. Evidently, heating treatment below gelatinization temperature was effective in enhancing the degree of hydrolysis of granular starch.
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spelling upm.eprints-145352015-11-04T03:16:56Z http://psasir.upm.edu.my/id/eprint/14535/ Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature. Yussof, Nor Shariffa Alias, Abd Karim Ariffin, Fazilah Sarker, Zaidul Islam The effect of mild heat treatment (below gelatinization temperature) towards the susceptibility of granular starch to enzymatic hydrolysis was investigated. Tapioca and sweet potato starches were subjected to enzymatic hydrolysis with a mixture of fungal α-amylase and glucoamylase at 35 °C for 24 h. Starches were hydrolyzed in native (granular) state and after heat treatment below gelatinization temperature (60 °C for 30 min). The dextrose equivalent (DE) value of heat-treated starch increased significantly compared to native starch, i.e., 36–50% and 27–34% for tapioca and sweet potato starch, respectively. Scanning electron microscopy examination showed that enzymatic erosion occurred mainly at the surface of starch granules. Hydrolyzed heat-treated starch exhibited rougher surface and porous granules compared to native starch. X-ray analysis suggested that enzymatic erosion preferentially occurred in amorphous areas of the granules. The amylose content, swelling power and solubility showed insignificant increase for both starches. Evidently, heating treatment below gelatinization temperature was effective in enhancing the degree of hydrolysis of granular starch. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14535/1/Enzymatic%20hydrolysis%20of%20granular%20native%20and%20mildly%20heat.pdf Yussof, Nor Shariffa and Alias, Abd Karim and Ariffin, Fazilah and Sarker, Zaidul Islam (2009) Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature. Food Hydrocolloids, 23 (2). pp. 434-440. ISSN 0268-005X; ESSN: 1873-7137 10.1016/j.foodhyd.2008.03.009 English
spellingShingle Yussof, Nor Shariffa
Alias, Abd Karim
Ariffin, Fazilah
Sarker, Zaidul Islam
Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.
title Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.
title_full Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.
title_fullStr Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.
title_full_unstemmed Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.
title_short Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature.
title_sort enzymatic hydrolysis of granular native and mildly heat treated tapioca and sweet potato starches at sub gelatinization temperature
url http://psasir.upm.edu.my/id/eprint/14535/1/Enzymatic%20hydrolysis%20of%20granular%20native%20and%20mildly%20heat.pdf
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AT ariffinfazilah enzymatichydrolysisofgranularnativeandmildlyheattreatedtapiocaandsweetpotatostarchesatsubgelatinizationtemperature
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