Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis.
Safe attenuation has been done on marine pathogen Vibrio alginolyticus using naturally acidified fructose against vibriosis. Attenuation was confirmed by injecting the attenuated bacterium into fish where the survival rate was 100% compared to 50% survival in fish injected with non-attenuated bacter...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Medwell Publishing
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14611/1/Evaluation%20of%20safe%20attenuated%20vibrio%20alginolyticus%20for%20oral%20vaccination%20of%20lates%20calcarifer%20against%20vibriosis.pdf |
| _version_ | 1825945461263958016 |
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| author | Idris, Nurul H. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha |
| author_facet | Idris, Nurul H. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha |
| author_sort | Idris, Nurul H. |
| collection | UPM |
| description | Safe attenuation has been done on marine pathogen Vibrio alginolyticus using naturally acidified fructose against vibriosis. Attenuation was confirmed by injecting the attenuated bacterium into fish where the survival rate was 100% compared to 50% survival in fish injected with non-attenuated bacteria. The attenuated bacterium was then evaluated for oral vaccination of Lates calcarifer (Asian seabass). Fish were fed with fish pellet incorporated with attenuated and non-attenuated bacterium of V. alginolyticus for 30 days. They were measured for serum antibody production by conventional agglutination titer and also monitored for the fish weight gain to observe the health improvement. Vaccinated fish showed comparable increased in weight gain, 90% survival after challenge and significantly high antibody titer compared to other treatment and control. |
| first_indexed | 2024-03-06T07:31:53Z |
| format | Article |
| id | upm.eprints-14611 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:31:53Z |
| publishDate | 2009 |
| publisher | Medwell Publishing |
| record_format | dspace |
| spelling | upm.eprints-146112015-09-29T07:45:40Z http://psasir.upm.edu.my/id/eprint/14611/ Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. Idris, Nurul H. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Safe attenuation has been done on marine pathogen Vibrio alginolyticus using naturally acidified fructose against vibriosis. Attenuation was confirmed by injecting the attenuated bacterium into fish where the survival rate was 100% compared to 50% survival in fish injected with non-attenuated bacteria. The attenuated bacterium was then evaluated for oral vaccination of Lates calcarifer (Asian seabass). Fish were fed with fish pellet incorporated with attenuated and non-attenuated bacterium of V. alginolyticus for 30 days. They were measured for serum antibody production by conventional agglutination titer and also monitored for the fish weight gain to observe the health improvement. Vaccinated fish showed comparable increased in weight gain, 90% survival after challenge and significantly high antibody titer compared to other treatment and control. Medwell Publishing 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14611/1/Evaluation%20of%20safe%20attenuated%20vibrio%20alginolyticus%20for%20oral%20vaccination%20of%20lates%20calcarifer%20against%20vibriosis.pdf Idris, Nurul H. and Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha (2009) Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. Research Journal of Biological Sciences, 4 (4). pp. 509-513. ISSN 1815-8846 rjbsci.2009.509.513 English |
| spellingShingle | Idris, Nurul H. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. |
| title | Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. |
| title_full | Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. |
| title_fullStr | Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. |
| title_full_unstemmed | Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. |
| title_short | Evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis. |
| title_sort | evaluation of safe attenuated vibrio alginolyticus for oral vaccination of lates calcarifer against vibriosis |
| url | http://psasir.upm.edu.my/id/eprint/14611/1/Evaluation%20of%20safe%20attenuated%20vibrio%20alginolyticus%20for%20oral%20vaccination%20of%20lates%20calcarifer%20against%20vibriosis.pdf |
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