Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide com...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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MDPI Publishing
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14671/1/Expression%20of%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf |
| _version_ | 1825945472815071232 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Yunus, Jalimah Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Yunus, Jalimah Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| collection | UPM |
| description | An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids. Expression of AT2 lipase resulted in an 18-fold increase in activity, upon the induction of 0.6 mM IPTG after a 10 h incubation period. Interestingly, this lipase was stable in various organic solvents (25% (v/v), mainly toluene, octanol, p-xylene and n-hexane). Literature shows that most of the organic solvent stable bacterial lipases were produced by Pseudomonas sp. and Bacillus sp., but very few from Staphylococcus sp. This lipase demonstrates great potential to be employed in various industrial applications. |
| first_indexed | 2024-03-06T07:32:04Z |
| format | Article |
| id | upm.eprints-14671 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:32:04Z |
| publishDate | 2010 |
| publisher | MDPI Publishing |
| record_format | dspace |
| spelling | upm.eprints-146712015-11-02T02:24:28Z http://psasir.upm.edu.my/id/eprint/14671/ Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. Raja Abdul Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Yunus, Jalimah Salleh, Abu Bakar Basri, Mahiran An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids. Expression of AT2 lipase resulted in an 18-fold increase in activity, upon the induction of 0.6 mM IPTG after a 10 h incubation period. Interestingly, this lipase was stable in various organic solvents (25% (v/v), mainly toluene, octanol, p-xylene and n-hexane). Literature shows that most of the organic solvent stable bacterial lipases were produced by Pseudomonas sp. and Bacillus sp., but very few from Staphylococcus sp. This lipase demonstrates great potential to be employed in various industrial applications. MDPI Publishing 2010-09-13 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14671/1/Expression%20of%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf Raja Abdul Rahman, Raja Noor Zaliha and Ahmad Kamarudin, Nor Hafizah and Yunus, Jalimah and Salleh, Abu Bakar and Basri, Mahiran (2010) Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. International Journal of Molecular Sciences, 11. pp. 3195-3208. ISSN 1422-0067 http://www.mdpi.com/journal/ijms 10.3390/ijms11093195 English |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Yunus, Jalimah Salleh, Abu Bakar Basri, Mahiran Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. |
| title | Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. |
| title_full | Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. |
| title_fullStr | Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. |
| title_full_unstemmed | Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. |
| title_short | Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. |
| title_sort | expression of organic solvent stable lipase from staphylococcus epidermidis at2 |
| url | http://psasir.upm.edu.my/id/eprint/14671/1/Expression%20of%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf |
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