Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation
A study was conducted to portray a preliminary characteristic of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli in a local tertiary hospital in Malaysia. Sixteen clinical isolates of ESBLs producing E. coli from different sources were examined for blaSHV/TEM/CTX-M ESBL genes by PC...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14675/1/14675.pdf |
| _version_ | 1796968692599226368 |
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| author | Sekawi, Zamberi Yusof, Rusmah Shamsudin, Mariana Nor |
| author_facet | Sekawi, Zamberi Yusof, Rusmah Shamsudin, Mariana Nor |
| author_sort | Sekawi, Zamberi |
| collection | UPM |
| description | A study was conducted to portray a preliminary characteristic of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli in a local tertiary hospital in Malaysia. Sixteen clinical isolates of ESBLs producing E. coli from different sources were examined for blaSHV/TEM/CTX-M ESBL genes by PCR molecular assay. Each strain was found to carry at least one of the genes. This study demonstrated a high prevalence of blaCTX-M (81.3%) and blaTEM genes (75%). Only two strains (12.5%) carried the blaSHV gene. Nucleotide and deduced protein sequences determination showed; 61% produced CTX-M-15, 31% produced CTX-M-14 and 8% produced CTX-M-3. The antimicrobial susceptibility testing data determined that almost all sixteen isolates were resistant to oxyimino-cephalosporins, 46% resistant to gentamicin, 69% resistant to trimethoprim-sulfamethoxazole and 46% resistant to ciprofloxacin. This study preliminary emphasizes the epidemiology of the ESBL-producing E. coli particularly SHV, TEM and CTX-M-type producing E. coli in Malaysia. |
| first_indexed | 2024-03-06T07:32:04Z |
| format | Article |
| id | upm.eprints-14675 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:32:04Z |
| publishDate | 2008 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-146752019-04-09T03:46:13Z http://psasir.upm.edu.my/id/eprint/14675/ Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation Sekawi, Zamberi Yusof, Rusmah Shamsudin, Mariana Nor A study was conducted to portray a preliminary characteristic of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli in a local tertiary hospital in Malaysia. Sixteen clinical isolates of ESBLs producing E. coli from different sources were examined for blaSHV/TEM/CTX-M ESBL genes by PCR molecular assay. Each strain was found to carry at least one of the genes. This study demonstrated a high prevalence of blaCTX-M (81.3%) and blaTEM genes (75%). Only two strains (12.5%) carried the blaSHV gene. Nucleotide and deduced protein sequences determination showed; 61% produced CTX-M-15, 31% produced CTX-M-14 and 8% produced CTX-M-3. The antimicrobial susceptibility testing data determined that almost all sixteen isolates were resistant to oxyimino-cephalosporins, 46% resistant to gentamicin, 69% resistant to trimethoprim-sulfamethoxazole and 46% resistant to ciprofloxacin. This study preliminary emphasizes the epidemiology of the ESBL-producing E. coli particularly SHV, TEM and CTX-M-type producing E. coli in Malaysia. Academic Journals 2008 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14675/1/14675.pdf Sekawi, Zamberi and Yusof, Rusmah and Shamsudin, Mariana Nor (2008) Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation. Research Journal of Microbiology, 3 (6). pp. 489-493. ISSN 1816-4935 https://scialert.net/abstract/?doi=jm.2008.489.493 10.3923/jm.2008.489.493 |
| spellingShingle | Sekawi, Zamberi Yusof, Rusmah Shamsudin, Mariana Nor Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation |
| title | Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation |
| title_full | Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation |
| title_fullStr | Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation |
| title_full_unstemmed | Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation |
| title_short | Extended-spectrum β-lactamases-producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX-M-type β-lactamases variation |
| title_sort | extended spectrum β lactamases producing escherichia coli from a tertiary hospital in malaysia emergence of ctx m type β lactamases variation |
| url | http://psasir.upm.edu.my/id/eprint/14675/1/14675.pdf |
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