Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers

Knowl­edge on genetic dis­tances and rela­tion­ships among breed­ing mate­ri­als has a sig­nif­i­cant impact on crop improve­ment. Mol­e­c­u­lar mark­ers are being used increas­ingly to deter­mine the genetic dis­tance between indi­vid­u­als. In this study, microsatel­lite mark­ers were used to esti...

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Main Authors: Abdullah, Norziha, Yusop, Mohd Rafii, Ithnin, Maizura, Saleh, Ghizan
Format: Article
Language:English
Published: Malaysian Palm Oil Board 2008
Online Access:http://psasir.upm.edu.my/id/eprint/14880/1/Genetic%20variation%20among%20oil%20palm%20parent%20genotypes%20and%20their%20progenies%20based%20on%20microsatellite%20markers.pdf
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author Abdullah, Norziha
Yusop, Mohd Rafii
Ithnin, Maizura
Saleh, Ghizan
author_facet Abdullah, Norziha
Yusop, Mohd Rafii
Ithnin, Maizura
Saleh, Ghizan
author_sort Abdullah, Norziha
collection UPM
description Knowl­edge on genetic dis­tances and rela­tion­ships among breed­ing mate­ri­als has a sig­nif­i­cant impact on crop improve­ment. Mol­e­c­u­lar mark­ers are being used increas­ingly to deter­mine the genetic dis­tance between indi­vid­u­als. In this study, microsatel­lite mark­ers were used to esti­mate the genetic dis­tances between selected oil palm par­ent lines. Microsatel­lite mark­ers are highly reli­able, inher­ited in codom­i­nant fash­ion whereby het­erozy­gotes and homozy­gotes are dis­tin­guish­able, easy to score and can be rapidly pro­duced using PCR tech­nol­ogy. Nine microsatel­lite mark­ers were used to screen selected par­ent palms (15 duras and 4 pisiferas) and their prog­e­nies (16 DxP crosses). Data were scored and analysed using the Biosys-1 soft­ware to cal­cu­late the genetic dis­tance val­ues. A total of 29 poly­mor­phic bands were gen­er­ated. The genetic dis­tances between prog­e­nies ranged from 0.089 to 0.313. These results indi­cate that microsatel­lite mark­ers are pow­er­ful tools for study­ing genetic rela­tion­ships among DxP prog­e­nies. These mark­ers should be fur­ther explored to assist oil palm breeding.
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spelling upm.eprints-148802015-12-01T07:19:49Z http://psasir.upm.edu.my/id/eprint/14880/ Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers Abdullah, Norziha Yusop, Mohd Rafii Ithnin, Maizura Saleh, Ghizan Knowl­edge on genetic dis­tances and rela­tion­ships among breed­ing mate­ri­als has a sig­nif­i­cant impact on crop improve­ment. Mol­e­c­u­lar mark­ers are being used increas­ingly to deter­mine the genetic dis­tance between indi­vid­u­als. In this study, microsatel­lite mark­ers were used to esti­mate the genetic dis­tances between selected oil palm par­ent lines. Microsatel­lite mark­ers are highly reli­able, inher­ited in codom­i­nant fash­ion whereby het­erozy­gotes and homozy­gotes are dis­tin­guish­able, easy to score and can be rapidly pro­duced using PCR tech­nol­ogy. Nine microsatel­lite mark­ers were used to screen selected par­ent palms (15 duras and 4 pisiferas) and their prog­e­nies (16 DxP crosses). Data were scored and analysed using the Biosys-1 soft­ware to cal­cu­late the genetic dis­tance val­ues. A total of 29 poly­mor­phic bands were gen­er­ated. The genetic dis­tances between prog­e­nies ranged from 0.089 to 0.313. These results indi­cate that microsatel­lite mark­ers are pow­er­ful tools for study­ing genetic rela­tion­ships among DxP prog­e­nies. These mark­ers should be fur­ther explored to assist oil palm breeding. Malaysian Palm Oil Board 2008-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14880/1/Genetic%20variation%20among%20oil%20palm%20parent%20genotypes%20and%20their%20progenies%20based%20on%20microsatellite%20markers.pdf Abdullah, Norziha and Yusop, Mohd Rafii and Ithnin, Maizura and Saleh, Ghizan (2008) Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers. Journal of Oil Palm Research, 20 (2). pp. 533-541. ISSN 1511-2780
spellingShingle Abdullah, Norziha
Yusop, Mohd Rafii
Ithnin, Maizura
Saleh, Ghizan
Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
title Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
title_full Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
title_fullStr Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
title_full_unstemmed Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
title_short Genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
title_sort genetic variation among oil palm parent genotypes and their progenies based on microsatellite markers
url http://psasir.upm.edu.my/id/eprint/14880/1/Genetic%20variation%20among%20oil%20palm%20parent%20genotypes%20and%20their%20progenies%20based%20on%20microsatellite%20markers.pdf
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AT ithninmaizura geneticvariationamongoilpalmparentgenotypesandtheirprogeniesbasedonmicrosatellitemarkers
AT salehghizan geneticvariationamongoilpalmparentgenotypesandtheirprogeniesbasedonmicrosatellitemarkers