Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia.
Pulse-Field Gel Electrophoresis (PFGE) and BOX-PCR patterns were used to determine the phylogenetic relationships among 32 strains of Ralstonia solanacearum collected from banana farms in West Malaysia. Eighty two percent (25 stains) were biovar 3 and the remainders were biovar 4. Cluster analysis b...
| Κύριοι συγγραφείς: | , , , , , |
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| Μορφή: | Άρθρο |
| Γλώσσα: | English English |
| Έκδοση: |
Asian Network for Scientific Information
2008
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| Διαθέσιμο Online: | http://psasir.upm.edu.my/id/eprint/14883/1/Genomic%20diversity%20of%20Ralstonia%20solanacearum%20strains%20isolated%20from%20banana%20farms%20in%20West%20Malaysia.pdf |
| _version_ | 1825945514261086208 |
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| author | Khakvar, R. Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Jones, J. Thong, K. L. |
| author_facet | Khakvar, R. Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Jones, J. Thong, K. L. |
| author_sort | Khakvar, R. |
| collection | UPM |
| description | Pulse-Field Gel Electrophoresis (PFGE) and BOX-PCR patterns were used to determine the phylogenetic relationships among 32 strains of Ralstonia solanacearum collected from banana farms in West Malaysia. Eighty two percent (25 stains) were biovar 3 and the remainders were biovar 4. Cluster analysis based on PFGE and BOX-PCR fingerprinting showed that there was significant genetic variation among all strains within this species in West Malaysia. Patterns of DNA fingerprinting in both methods were significantly correlated with sampling sites and revealed the regional similarity among the strains. The PFGE patterns varied from 78 to 99% while BOX-PCR patterns showed a high degree of variation among R. solanacearum strains. Genomic fingerprinting by PFGE also revealed one that was significantly correlated to biovar type while BOX-PCR pattern analysis was unable to differentiate various biovars. This study clearly showed that R. solanacearum strains were phylogenetically similar within a region but diverse between regions despite biovar designation. |
| first_indexed | 2024-03-06T07:32:42Z |
| format | Article |
| id | upm.eprints-14883 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:32:42Z |
| publishDate | 2008 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | upm.eprints-148832015-09-28T07:54:48Z http://psasir.upm.edu.my/id/eprint/14883/ Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. Khakvar, R. Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Jones, J. Thong, K. L. Pulse-Field Gel Electrophoresis (PFGE) and BOX-PCR patterns were used to determine the phylogenetic relationships among 32 strains of Ralstonia solanacearum collected from banana farms in West Malaysia. Eighty two percent (25 stains) were biovar 3 and the remainders were biovar 4. Cluster analysis based on PFGE and BOX-PCR fingerprinting showed that there was significant genetic variation among all strains within this species in West Malaysia. Patterns of DNA fingerprinting in both methods were significantly correlated with sampling sites and revealed the regional similarity among the strains. The PFGE patterns varied from 78 to 99% while BOX-PCR patterns showed a high degree of variation among R. solanacearum strains. Genomic fingerprinting by PFGE also revealed one that was significantly correlated to biovar type while BOX-PCR pattern analysis was unable to differentiate various biovars. This study clearly showed that R. solanacearum strains were phylogenetically similar within a region but diverse between regions despite biovar designation. Asian Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14883/1/Genomic%20diversity%20of%20Ralstonia%20solanacearum%20strains%20isolated%20from%20banana%20farms%20in%20West%20Malaysia.pdf Khakvar, R. and Sijam, Kamaruzaman and Wong, Mui Yun and Radu, Son and Jones, J. and Thong, K. L. (2008) Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. Plant Pathology Journal, 7 (2). pp. 162-167. ISSN 1812-5425 English |
| spellingShingle | Khakvar, R. Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Jones, J. Thong, K. L. Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. |
| title | Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. |
| title_full | Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. |
| title_fullStr | Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. |
| title_full_unstemmed | Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. |
| title_short | Genomic diversity of Ralstonia solanacearum strains isolated from banana farms in West Malaysia. |
| title_sort | genomic diversity of ralstonia solanacearum strains isolated from banana farms in west malaysia |
| url | http://psasir.upm.edu.my/id/eprint/14883/1/Genomic%20diversity%20of%20Ralstonia%20solanacearum%20strains%20isolated%20from%20banana%20farms%20in%20West%20Malaysia.pdf |
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