Haematological parameters of leukaemic rats supplemented with Morinda citrifolia
Morinda citrifolia (mengkudu) had been reported to have anti-tumor activity, which has been researched widely in several animal models. Sixty four eight-week-old male Sprague Dawley rats were divided into four groups of 16 rats per group namely control, MNU, Morinda citrifolia (MC), and MC+MNU group...
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| Format: | Article |
| Language: | English |
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Malaysian Medical Association
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14956/1/Haematological%20parameters%20of%20leukaemic%20rats%20supplemented%20with%20Morinda%20citrifolia.pdf |
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| author | Hamzah, Hazilawati Al-Salih, Hutheyfa A. H. Shaari, Mohd Rosly Sabri, Jasni Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu |
| author_facet | Hamzah, Hazilawati Al-Salih, Hutheyfa A. H. Shaari, Mohd Rosly Sabri, Jasni Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu |
| author_sort | Hamzah, Hazilawati |
| collection | UPM |
| description | Morinda citrifolia (mengkudu) had been reported to have anti-tumor activity, which has been researched widely in several animal models. Sixty four eight-week-old male Sprague Dawley rats were divided into four groups of 16 rats per group namely control, MNU, Morinda citrifolia (MC), and MC+MNU group. The MNU and MC+MNU groups received four consecutive intraperitoneal (i.p) injections of N-methyl-N-nitrosourea (MNU) at a dose of 60 mg/kg for induction of leukaemia. Rats in the MC and MC+MNU groups were fed daily with a ration mixed with M. citrifolia at a dose of 5000 mg/kg body weight. The peripheral blood samples were collected at 20 weeks post MNU administration into EDTA tubes and analysed for a complete blood count. Blood smears stained with Wright’s stain were prepared for a manual differential leukocyte count and examination of the leukaemic cells. The results were analysed using a one-way ANOVA. Results in this study showed that MNU group had significant lymphocytosis (66.9±98.14) compared to the other groups. The morphology of the lymphocytes in the MNU and MC+MNU groups showed a typical morphology of leukaemic cell, while the other groups had normal lymphocyte morphology. Rats in the MNU group also had anaemia with significant reduction in total erythrocyte number (6.11±2.73 x 1012/L), haemoglobin concentration (131.64±21.32 g/L) and packed cell volume (37.92±8.50 L/L). The erythron parameters of MC+MNU group were comparable to the control and MC groups. In conclusion, daily supplementation of M. citrifolia reduced the proliferation of circulating leukaemic cells. |
| first_indexed | 2024-03-06T07:32:54Z |
| format | Article |
| id | upm.eprints-14956 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:32:54Z |
| publishDate | 2010 |
| publisher | Malaysian Medical Association |
| record_format | dspace |
| spelling | upm.eprints-149562016-11-21T08:39:22Z http://psasir.upm.edu.my/id/eprint/14956/ Haematological parameters of leukaemic rats supplemented with Morinda citrifolia Hamzah, Hazilawati Al-Salih, Hutheyfa A. H. Shaari, Mohd Rosly Sabri, Jasni Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Morinda citrifolia (mengkudu) had been reported to have anti-tumor activity, which has been researched widely in several animal models. Sixty four eight-week-old male Sprague Dawley rats were divided into four groups of 16 rats per group namely control, MNU, Morinda citrifolia (MC), and MC+MNU group. The MNU and MC+MNU groups received four consecutive intraperitoneal (i.p) injections of N-methyl-N-nitrosourea (MNU) at a dose of 60 mg/kg for induction of leukaemia. Rats in the MC and MC+MNU groups were fed daily with a ration mixed with M. citrifolia at a dose of 5000 mg/kg body weight. The peripheral blood samples were collected at 20 weeks post MNU administration into EDTA tubes and analysed for a complete blood count. Blood smears stained with Wright’s stain were prepared for a manual differential leukocyte count and examination of the leukaemic cells. The results were analysed using a one-way ANOVA. Results in this study showed that MNU group had significant lymphocytosis (66.9±98.14) compared to the other groups. The morphology of the lymphocytes in the MNU and MC+MNU groups showed a typical morphology of leukaemic cell, while the other groups had normal lymphocyte morphology. Rats in the MNU group also had anaemia with significant reduction in total erythrocyte number (6.11±2.73 x 1012/L), haemoglobin concentration (131.64±21.32 g/L) and packed cell volume (37.92±8.50 L/L). The erythron parameters of MC+MNU group were comparable to the control and MC groups. In conclusion, daily supplementation of M. citrifolia reduced the proliferation of circulating leukaemic cells. Malaysian Medical Association 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14956/1/Haematological%20parameters%20of%20leukaemic%20rats%20supplemented%20with%20Morinda%20citrifolia.pdf Hamzah, Hazilawati and Al-Salih, Hutheyfa A. H. and Shaari, Mohd Rosly and Sabri, Jasni and Mohamed Mustapha, Noordin and Sithambaram, Shanmugavelu (2010) Haematological parameters of leukaemic rats supplemented with Morinda citrifolia. Medical Journal of Malaysia, 65 (suppl.B). pp. 125-126. ISSN 0300-5283 http://www.e-mjm.org/2010/Sup_tissue_engineering_content.pdf |
| spellingShingle | Hamzah, Hazilawati Al-Salih, Hutheyfa A. H. Shaari, Mohd Rosly Sabri, Jasni Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haematological parameters of leukaemic rats supplemented with Morinda citrifolia |
| title | Haematological parameters of leukaemic rats supplemented with Morinda citrifolia |
| title_full | Haematological parameters of leukaemic rats supplemented with Morinda citrifolia |
| title_fullStr | Haematological parameters of leukaemic rats supplemented with Morinda citrifolia |
| title_full_unstemmed | Haematological parameters of leukaemic rats supplemented with Morinda citrifolia |
| title_short | Haematological parameters of leukaemic rats supplemented with Morinda citrifolia |
| title_sort | haematological parameters of leukaemic rats supplemented with morinda citrifolia |
| url | http://psasir.upm.edu.my/id/eprint/14956/1/Haematological%20parameters%20of%20leukaemic%20rats%20supplemented%20with%20Morinda%20citrifolia.pdf |
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