Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus
A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 deg...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier B.V.
2010
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/14999/1/Hexavalent%20molybdenum%20reduction%20to%20mo.pdf |
| _version_ | 1796968758322921472 |
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| author | Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif |
| author_facet | Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif |
| author_sort | Abd. Shukor , Mohd. Yunus |
| collection | UPM |
| description | A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. |
| first_indexed | 2024-03-06T07:33:02Z |
| format | Article |
| id | upm.eprints-14999 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:33:02Z |
| publishDate | 2010 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | upm.eprints-149992015-10-29T07:56:59Z http://psasir.upm.edu.my/id/eprint/14999/ Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif A local molybdenum-reducing bacterium was isolated and tentatively identified as Acinetobacter calcoaceticus strain Dr.Y12 based on carbon utilization profiles using Biolog GN plates and 16S rDNA comparative analysis. Molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees C and pH 6.5). Of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest Mo-blue production. Optimum Mo-blue production was reached at 20 mmol/L molybdate and 5 mmol/L phosphate; increasing the phosphate concentrations inhibited the production. An increase in an overall absorption profiles, especially at peak maximum at 865 nm and the shoulder at 700 nm, was observed in direct correlation with the increased in Mo-blue amounts. Metal ions, such as chromium, cadmium, copper, mercury and lead (2 mmol/L final concentration) caused approximately 88, 53, 80, 100, and 20 % inhibition, respectively. Respiratory inhibitors, such as antimycin A, rotenone, sodium azide and cyanide showed in this bacterium no inhibition of the Mo-blue production, suggesting that the electron transport system is not a site of molybdate reduction. Elsevier B.V. 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14999/1/Hexavalent%20molybdenum%20reduction%20to%20mo.pdf Abd. Shukor , Mohd. Yunus and Rahman, MF and Suhaili, Zarizal and Shamaan, Nor Aripin and Syed, Mohd. Arif (2010) Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus. Folia microbiologica, 55 (2). pp. 137-143. ISSN 0015-5632 Molybdenum - Analysis Acinetobacter DOI: 10.1007/s12223-010-0021-x English |
| spellingShingle | Molybdenum - Analysis Acinetobacter Abd. Shukor , Mohd. Yunus Rahman, MF Suhaili, Zarizal Shamaan, Nor Aripin Syed, Mohd. Arif Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |
| title | Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |
| title_full | Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |
| title_fullStr | Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |
| title_full_unstemmed | Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |
| title_short | Hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus |
| title_sort | hexavalent molybdenum reduction to mo blue by acinetobacter calcoaceticus |
| topic | Molybdenum - Analysis Acinetobacter |
| url | http://psasir.upm.edu.my/id/eprint/14999/1/Hexavalent%20molybdenum%20reduction%20to%20mo.pdf |
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