Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling
Neobalanocarpus heimii, locally known as chengal, is an important timber species in Peninsular Malaysia. Owing to the high demand for its valuable timber, N. heimii is subjected to illegal logging and this species may become endangered in the near future. The present study was designed to identify a...
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| Format: | Article |
| Language: | English |
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Forest Research Institute Malaysia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15014/1/Highly%20variable%20STR%20markers%20of%20Neobalanocarpus%20heimii.pdf |
| _version_ | 1825945539078782976 |
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| author | Tnah, Lee Hong Lee, Soon Leong Ng, Kevin Kit Siong Qamaruz Zaman, Faridah Ibrahim, Faridah Hanum |
| author_facet | Tnah, Lee Hong Lee, Soon Leong Ng, Kevin Kit Siong Qamaruz Zaman, Faridah Ibrahim, Faridah Hanum |
| author_sort | Tnah, Lee Hong |
| collection | UPM |
| description | Neobalanocarpus heimii, locally known as chengal, is an important timber species in Peninsular Malaysia. Owing to the high demand for its valuable timber, N. heimii is subjected to illegal logging and this species may become endangered in the near future. The present study was designed to identify a set of highly polymorphic short tandem repeat (STR) markers for timber tracking of N. heimii. An extensive evaluation of 51 STRs developed for Dipterocarpaceae managed to identify 12 STR loci (Nhe004, Nhe005, Nhe011, Nhe015, Nhe018, Hbi161, Sle392, Sle605, Slu044a, Shc03, Shc04 and Shc07), which showed specific amplification, high polymorphism, single-locus mode of inheritance, absence of null alleles and absence of mononucleotide repeat motifs in N. heimii. These loci can be readily used to establish a linkage between the evidentiary sample and the source, thus providing a useful set of markers for individual identification in N. heimii. |
| first_indexed | 2024-03-06T07:33:05Z |
| format | Article |
| id | upm.eprints-15014 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:33:05Z |
| publishDate | 2010 |
| publisher | Forest Research Institute Malaysia |
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| spelling | upm.eprints-150142016-08-22T08:36:26Z http://psasir.upm.edu.my/id/eprint/15014/ Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling Tnah, Lee Hong Lee, Soon Leong Ng, Kevin Kit Siong Qamaruz Zaman, Faridah Ibrahim, Faridah Hanum Neobalanocarpus heimii, locally known as chengal, is an important timber species in Peninsular Malaysia. Owing to the high demand for its valuable timber, N. heimii is subjected to illegal logging and this species may become endangered in the near future. The present study was designed to identify a set of highly polymorphic short tandem repeat (STR) markers for timber tracking of N. heimii. An extensive evaluation of 51 STRs developed for Dipterocarpaceae managed to identify 12 STR loci (Nhe004, Nhe005, Nhe011, Nhe015, Nhe018, Hbi161, Sle392, Sle605, Slu044a, Shc03, Shc04 and Shc07), which showed specific amplification, high polymorphism, single-locus mode of inheritance, absence of null alleles and absence of mononucleotide repeat motifs in N. heimii. These loci can be readily used to establish a linkage between the evidentiary sample and the source, thus providing a useful set of markers for individual identification in N. heimii. Forest Research Institute Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15014/1/Highly%20variable%20STR%20markers%20of%20Neobalanocarpus%20heimii.pdf Tnah, Lee Hong and Lee, Soon Leong and Ng, Kevin Kit Siong and Qamaruz Zaman, Faridah and Ibrahim, Faridah Hanum (2010) Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling. Journal of Tropical Forest Science, 22 (2). pp. 214-226. ISSN 0128-1283 https://www.frim.gov.my/publication/journal-of-tropical-forest-science-jtfs/ |
| spellingShingle | Tnah, Lee Hong Lee, Soon Leong Ng, Kevin Kit Siong Qamaruz Zaman, Faridah Ibrahim, Faridah Hanum Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling |
| title | Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling |
| title_full | Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling |
| title_fullStr | Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling |
| title_full_unstemmed | Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling |
| title_short | Highly variable STR markers of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling |
| title_sort | highly variable str markers of neobalanocarpus heimii dipterocarpaceae for forensic dna profiling |
| url | http://psasir.upm.edu.my/id/eprint/15014/1/Highly%20variable%20STR%20markers%20of%20Neobalanocarpus%20heimii.pdf |
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