Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris
Univariate screening on factors affecting the purification performance of recombinant hepatitis B surface antigen (HBsAg) on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) and the establishment of a two-step purification strategy were performed. Amongst four IEC adsorbents...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15080/1/Two-step%20purification%20strategy%20for%20enhanced%20recovery%20of%20recombinant%20hepatitis%20B%20surface%20antigen%20from%20Pichia%20pastoris.pdf |
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| author | Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun |
| author_facet | Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun |
| author_sort | Tam, Yew Joon |
| collection | UPM |
| description | Univariate screening on factors affecting the purification performance of recombinant hepatitis B surface antigen (HBsAg) on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) and the establishment of a two-step purification strategy were performed. Amongst four IEC adsorbents examined, the use of Q Sepharose XL IEC adsorbent under optimized conditions together with optimized SEC purification was able to efficiently purify HBsAg. An established purification strategy comprising the two techniques further demonstrated adaptability for scale-up operations with a final total purification factor (PF) of 94.82 ± 16.20, HBsAg purity of 95.48% and recovery yield of 78.07%. |
| first_indexed | 2024-03-06T07:33:17Z |
| format | Article |
| id | upm.eprints-15080 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:33:17Z |
| publishDate | 2016 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-150802019-05-09T01:23:05Z http://psasir.upm.edu.my/id/eprint/15080/ Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Univariate screening on factors affecting the purification performance of recombinant hepatitis B surface antigen (HBsAg) on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) and the establishment of a two-step purification strategy were performed. Amongst four IEC adsorbents examined, the use of Q Sepharose XL IEC adsorbent under optimized conditions together with optimized SEC purification was able to efficiently purify HBsAg. An established purification strategy comprising the two techniques further demonstrated adaptability for scale-up operations with a final total purification factor (PF) of 94.82 ± 16.20, HBsAg purity of 95.48% and recovery yield of 78.07%. Taylor & Francis 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15080/1/Two-step%20purification%20strategy%20for%20enhanced%20recovery%20of%20recombinant%20hepatitis%20B%20surface%20antigen%20from%20Pichia%20pastoris.pdf Tam, Yew Joon and Allaudin, Zeenathul Nazariah and Rezaei, Morvarid Akhavan and Mohd Lila, Mohd Azmi and Bahaman, Abdul Rani and Lo, Sewn Cen and Tan, Joo Shun and Hani, Homayoun (2016) Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris. Separation Science and Technology, 51 (5). pp. 816-829. ISSN 0149-6395; ESSN: 1520-5754 https://www.tandfonline.com/doi/abs/10.1080/01496395.2015.1135949 10.1080/01496395.2015.1135949 |
| spellingShingle | Tam, Yew Joon Allaudin, Zeenathul Nazariah Rezaei, Morvarid Akhavan Mohd Lila, Mohd Azmi Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris |
| title | Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris |
| title_full | Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris |
| title_fullStr | Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris |
| title_full_unstemmed | Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris |
| title_short | Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris |
| title_sort | two step purification strategy for enhanced recovery of recombinant hepatitis b surface antigen from pichia pastoris |
| url | http://psasir.upm.edu.my/id/eprint/15080/1/Two-step%20purification%20strategy%20for%20enhanced%20recovery%20of%20recombinant%20hepatitis%20B%20surface%20antigen%20from%20Pichia%20pastoris.pdf |
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