Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm.
Introduction: Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence...
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| Format: | Article |
| Language: | English |
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Medknow Publications
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15100/1/Immunohistochemical%20study%20of%20p16INK4A%20and%20survivin%20expressions%20in%20cervical%20squamous%20neoplasm.pdf |
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| author | Tan, Geok Chin Sydee, Norlatiffah Syed Husain Shahabuddin, Sharifah Noor Akmal Ghazali, Razmin Mohd Sidik, Shiran Mohd Dali, Ahmad Zailani Hatta Ng, Paul Hock Oon |
| author_facet | Tan, Geok Chin Sydee, Norlatiffah Syed Husain Shahabuddin, Sharifah Noor Akmal Ghazali, Razmin Mohd Sidik, Shiran Mohd Dali, Ahmad Zailani Hatta Ng, Paul Hock Oon |
| author_sort | Tan, Geok Chin |
| collection | UPM |
| description | Introduction:
Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence the need to search for novel biomarkers to assist in the screening and diagnosis of cervical neoplasia. This study aims to determine the expression of p16 INK4A and survivin as possible predictive biomarkers in cervical squamous neoplasm.
Material and Methods:
This is a retrospective study on 201 cases of cervical neoplasm comprising of 129 cervical intraepithelial neoplasia (CIN) and 72 squamous cell carcinoma (SCC). All samples were evaluated by two independent observers using p16INK4A and survivin monoclonal antibodies. The p16INK4A expression was graded as negative, focal and diffuse positivity. The intensity for survivin expression was graded as weak, moderate and intense.
Results:
It is seen that p16INK4A expression in CIN 1, CIN 2 and CIN 3 were 25.4%, 42.9% and 95.9% respectively. Majority of SCC (98.6%) showed p16INK4A expression. Survivin expressions in CIN 1, CIN 2, CIN 3 and SCC were 56.7%, 33.4%, 87.5% and 98.6%. There was a linear relationship between increasing grade of CIN and p16INK4A expressions.
Conclusion:
Our study showed that p16INK4A expressions correlate well with the increasing grade of CIN. Although survivin does not correlate well to the increasing grade of CIN, it could be useful in differentiating CIN 3 from SCC. |
| first_indexed | 2024-03-06T07:33:20Z |
| format | Article |
| id | upm.eprints-15100 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:33:20Z |
| publishDate | 2010 |
| publisher | Medknow Publications |
| record_format | dspace |
| spelling | upm.eprints-151002019-11-27T07:58:16Z http://psasir.upm.edu.my/id/eprint/15100/ Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. Tan, Geok Chin Sydee, Norlatiffah Syed Husain Shahabuddin, Sharifah Noor Akmal Ghazali, Razmin Mohd Sidik, Shiran Mohd Dali, Ahmad Zailani Hatta Ng, Paul Hock Oon Introduction: Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence the need to search for novel biomarkers to assist in the screening and diagnosis of cervical neoplasia. This study aims to determine the expression of p16 INK4A and survivin as possible predictive biomarkers in cervical squamous neoplasm. Material and Methods: This is a retrospective study on 201 cases of cervical neoplasm comprising of 129 cervical intraepithelial neoplasia (CIN) and 72 squamous cell carcinoma (SCC). All samples were evaluated by two independent observers using p16INK4A and survivin monoclonal antibodies. The p16INK4A expression was graded as negative, focal and diffuse positivity. The intensity for survivin expression was graded as weak, moderate and intense. Results: It is seen that p16INK4A expression in CIN 1, CIN 2 and CIN 3 were 25.4%, 42.9% and 95.9% respectively. Majority of SCC (98.6%) showed p16INK4A expression. Survivin expressions in CIN 1, CIN 2, CIN 3 and SCC were 56.7%, 33.4%, 87.5% and 98.6%. There was a linear relationship between increasing grade of CIN and p16INK4A expressions. Conclusion: Our study showed that p16INK4A expressions correlate well with the increasing grade of CIN. Although survivin does not correlate well to the increasing grade of CIN, it could be useful in differentiating CIN 3 from SCC. Medknow Publications 2010-01-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15100/1/Immunohistochemical%20study%20of%20p16INK4A%20and%20survivin%20expressions%20in%20cervical%20squamous%20neoplasm.pdf Tan, Geok Chin and Sydee, Norlatiffah and Syed Husain Shahabuddin, Sharifah Noor Akmal and Ghazali, Razmin and Mohd Sidik, Shiran and Mohd Dali, Ahmad Zailani Hatta and Ng, Paul Hock Oon (2010) Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. Indian Journal of Pathology and Microbiology, 53 (1). pp. 1-6. ISSN 0377-4929; ESSN: 0974-5130 http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2010;volume=53;issue=1;spage=1;epage=6;aulast=Tan 10.4103/0377-4929.59173 |
| spellingShingle | Tan, Geok Chin Sydee, Norlatiffah Syed Husain Shahabuddin, Sharifah Noor Akmal Ghazali, Razmin Mohd Sidik, Shiran Mohd Dali, Ahmad Zailani Hatta Ng, Paul Hock Oon Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. |
| title | Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. |
| title_full | Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. |
| title_fullStr | Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. |
| title_full_unstemmed | Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. |
| title_short | Immunohistochemical study of p16INK4A and survivin expressions in cervical squamous neoplasm. |
| title_sort | immunohistochemical study of p16ink4a and survivin expressions in cervical squamous neoplasm |
| url | http://psasir.upm.edu.my/id/eprint/15100/1/Immunohistochemical%20study%20of%20p16INK4A%20and%20survivin%20expressions%20in%20cervical%20squamous%20neoplasm.pdf |
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