In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei.
Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core ohgosaccharide and O-s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Medwell Publishing
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15150/1/In%20silico%20annotation%20of%20the%20genes%20involved%20in%20biosynthesis%20of%20lipopolysaccharide%20for%20Burkholderia%20pseudomallei.pdf |
| _version_ | 1796968788609990656 |
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| author | Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah |
| author_facet | Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah |
| author_sort | Lai, L. Suang |
| collection | UPM |
| description | Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core ohgosaccharide and O-specific antigen. In the current study, by using the available B. pseudomallei genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in Burkholderia pseudomallei. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for B. pseudomallei and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen. |
| first_indexed | 2024-03-06T07:33:29Z |
| format | Article |
| id | upm.eprints-15150 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:33:29Z |
| publishDate | 2009 |
| publisher | Medwell Publishing |
| record_format | dspace |
| spelling | upm.eprints-151502015-10-05T06:35:27Z http://psasir.upm.edu.my/id/eprint/15150/ In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core ohgosaccharide and O-specific antigen. In the current study, by using the available B. pseudomallei genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in Burkholderia pseudomallei. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for B. pseudomallei and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen. Medwell Publishing 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15150/1/In%20silico%20annotation%20of%20the%20genes%20involved%20in%20biosynthesis%20of%20lipopolysaccharide%20for%20Burkholderia%20pseudomallei.pdf Lai, L. Suang and Sekawi, Zamberi and Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdullah, Rasedee and Mohamed, Rahmah (2009) In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. Research Journal of Applied Sciences, 4 (2). pp. 62-72. ISSN 1815-932X; ESSN : 1993-6079 http://www.medwelljournals.com English |
| spellingShingle | Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. |
| title | In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. |
| title_full | In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. |
| title_fullStr | In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. |
| title_full_unstemmed | In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. |
| title_short | In silico annotation of the genes involved in biosynthesis of lipopolysaccharide for Burkholderia pseudomallei. |
| title_sort | in silico annotation of the genes involved in biosynthesis of lipopolysaccharide for burkholderia pseudomallei |
| url | http://psasir.upm.edu.my/id/eprint/15150/1/In%20silico%20annotation%20of%20the%20genes%20involved%20in%20biosynthesis%20of%20lipopolysaccharide%20for%20Burkholderia%20pseudomallei.pdf |
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