In vitro culture of Borneo's endemic orchid, Vanda dearei
The effect of basal media, complex additives and sugars to the growth and development of V. dearei protocorms were reported in this study. Protocorms were initiated from in vitro seeds germination from immature capsule. Among the three basal media, growth of protocorms was superior in ½ MS medium f...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15162/1/In%20vitro%20culture%20of%20Borneo.pdf |
| _version_ | 1796968790965092352 |
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| author | Jawan, Roslina Gansau, Jualang @ Azlan Abdullah Abdullah, Janna Ong |
| author_facet | Jawan, Roslina Gansau, Jualang @ Azlan Abdullah Abdullah, Janna Ong |
| author_sort | Jawan, Roslina |
| collection | UPM |
| description | The effect of basal media, complex additives and sugars to the growth and development of V. dearei protocorms were
reported in this study. Protocorms were initiated from in vitro seeds germination from immature capsule. Among the three basal media, growth of protocorms was superior in ½ MS medium followed by KC and VW with maximum percentage of protocorms with leaf and root, and mean number of leaf and root produced. Meanwhile, 0.2% (w/v) yeast extract markedly enhanced the growth of protocorms. In sugar study, results show that protocorms preferred sucrose as compared to fructose and glucose. The leaf and root formation was significantly enhanced as the sugar concentration was decreased. Medium supplemented with 2%(w/v) sucrose was the best compared to the other treatments and sugar at a concentration of 4% (w/v) induced the formation of large size seedlings. |
| first_indexed | 2024-03-06T07:33:32Z |
| format | Article |
| id | upm.eprints-15162 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:33:32Z |
| publishDate | 2010 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-151622018-01-19T08:16:58Z http://psasir.upm.edu.my/id/eprint/15162/ In vitro culture of Borneo's endemic orchid, Vanda dearei Jawan, Roslina Gansau, Jualang @ Azlan Abdullah Abdullah, Janna Ong The effect of basal media, complex additives and sugars to the growth and development of V. dearei protocorms were reported in this study. Protocorms were initiated from in vitro seeds germination from immature capsule. Among the three basal media, growth of protocorms was superior in ½ MS medium followed by KC and VW with maximum percentage of protocorms with leaf and root, and mean number of leaf and root produced. Meanwhile, 0.2% (w/v) yeast extract markedly enhanced the growth of protocorms. In sugar study, results show that protocorms preferred sucrose as compared to fructose and glucose. The leaf and root formation was significantly enhanced as the sugar concentration was decreased. Medium supplemented with 2%(w/v) sucrose was the best compared to the other treatments and sugar at a concentration of 4% (w/v) induced the formation of large size seedlings. Malaysian Society for Molecular Biology and Biotechnology 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15162/1/In%20vitro%20culture%20of%20Borneo.pdf Jawan, Roslina and Gansau, Jualang @ Azlan Abdullah and Abdullah, Janna Ong (2010) In vitro culture of Borneo's endemic orchid, Vanda dearei. Asia Pacific Journal of Molecular Biology and Biotechnology, 18 (1). pp. 203-207. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html181/181av.htm |
| spellingShingle | Jawan, Roslina Gansau, Jualang @ Azlan Abdullah Abdullah, Janna Ong In vitro culture of Borneo's endemic orchid, Vanda dearei |
| title | In vitro culture of Borneo's endemic orchid, Vanda dearei |
| title_full | In vitro culture of Borneo's endemic orchid, Vanda dearei |
| title_fullStr | In vitro culture of Borneo's endemic orchid, Vanda dearei |
| title_full_unstemmed | In vitro culture of Borneo's endemic orchid, Vanda dearei |
| title_short | In vitro culture of Borneo's endemic orchid, Vanda dearei |
| title_sort | in vitro culture of borneo s endemic orchid vanda dearei |
| url | http://psasir.upm.edu.my/id/eprint/15162/1/In%20vitro%20culture%20of%20Borneo.pdf |
| work_keys_str_mv | AT jawanroslina invitrocultureofborneosendemicorchidvandadearei AT gansaujualangazlanabdullah invitrocultureofborneosendemicorchidvandadearei AT abdullahjannaong invitrocultureofborneosendemicorchidvandadearei |