In vivo anti-tumor effects of Azadirachta indica in rat liver cancer.
The aim of the current study is to determine the effects of A. indica aqueous extract on Diethyl Nitrosamine (DEN) and 2-Acetylaminofluorene (AAF) induced-hepatocarcinogenesis on Spraque-Dawley rats. The plant, A. indica, extract was prepared into 5% w/v in distilled water. Spraque-Dawley male rats...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Medwell Online.
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15176/1/In%20vivo%20anti.pdf |
| _version_ | 1825945570441691136 |
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| author | Mohamed Elhassan Taha , Manal Abdel Wahab , Siddig Ibrahim Othman, Fauziah Parichehr, Hanachi Abdul, Ahmad Bustamam Sharaf Al-Zubairi, Adel |
| author_facet | Mohamed Elhassan Taha , Manal Abdel Wahab , Siddig Ibrahim Othman, Fauziah Parichehr, Hanachi Abdul, Ahmad Bustamam Sharaf Al-Zubairi, Adel |
| author_sort | Mohamed Elhassan Taha , Manal |
| collection | UPM |
| description | The aim of the current study is to determine the effects of A. indica aqueous extract on Diethyl Nitrosamine (DEN) and 2-Acetylaminofluorene (AAF) induced-hepatocarcinogenesis on Spraque-Dawley rats. The plant, A. indica, extract was prepared into 5% w/v in distilled water. Spraque-Dawley male rats were divided into 3 groups of 7 rats each. The groups were: DEN/AAF-induced rats (C), DEN/AAF-induced rats treated with 5% A. indica (CAI) and normal control group (N). In situ detection of DNA fragmentation, TUNEL assay, was used to investigate the apoptogenic properties of A. indica. RT-PCR was used to amplify AFP mRNA. TUNEL assay supported that there was more numbers of apoptotic cells in the liver of (CAI) group compared with (C) group. AFP gene was suppressed by the supplementation of A. indica to DEN/AAF rats (CAI). A. indica (Neem) has revealed a chemopreventive capability by regressing the hepatocarcinogenesis induced by DEN/AAF carcinogens. This capability can be seen from the modulating effects of the plant in the biological indicators used in this study. |
| first_indexed | 2024-03-06T07:33:34Z |
| format | Article |
| id | upm.eprints-15176 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:33:34Z |
| publishDate | 2009 |
| publisher | Medwell Online. |
| record_format | dspace |
| spelling | upm.eprints-151762015-10-22T07:44:10Z http://psasir.upm.edu.my/id/eprint/15176/ In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. Mohamed Elhassan Taha , Manal Abdel Wahab , Siddig Ibrahim Othman, Fauziah Parichehr, Hanachi Abdul, Ahmad Bustamam Sharaf Al-Zubairi, Adel The aim of the current study is to determine the effects of A. indica aqueous extract on Diethyl Nitrosamine (DEN) and 2-Acetylaminofluorene (AAF) induced-hepatocarcinogenesis on Spraque-Dawley rats. The plant, A. indica, extract was prepared into 5% w/v in distilled water. Spraque-Dawley male rats were divided into 3 groups of 7 rats each. The groups were: DEN/AAF-induced rats (C), DEN/AAF-induced rats treated with 5% A. indica (CAI) and normal control group (N). In situ detection of DNA fragmentation, TUNEL assay, was used to investigate the apoptogenic properties of A. indica. RT-PCR was used to amplify AFP mRNA. TUNEL assay supported that there was more numbers of apoptotic cells in the liver of (CAI) group compared with (C) group. AFP gene was suppressed by the supplementation of A. indica to DEN/AAF rats (CAI). A. indica (Neem) has revealed a chemopreventive capability by regressing the hepatocarcinogenesis induced by DEN/AAF carcinogens. This capability can be seen from the modulating effects of the plant in the biological indicators used in this study. Medwell Online. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15176/1/In%20vivo%20anti.pdf Mohamed Elhassan Taha , Manal and Abdel Wahab , Siddig Ibrahim and Othman, Fauziah and Parichehr, Hanachi and Abdul, Ahmad Bustamam and Sharaf Al-Zubairi, Adel (2009) In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. Research Journal of Biological Sciences, 4 (1). pp. 48-53. ISSN 1815-8846, ESSN: 1993-6087 http://www.medwelljournals.com English |
| spellingShingle | Mohamed Elhassan Taha , Manal Abdel Wahab , Siddig Ibrahim Othman, Fauziah Parichehr, Hanachi Abdul, Ahmad Bustamam Sharaf Al-Zubairi, Adel In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. |
| title | In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. |
| title_full | In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. |
| title_fullStr | In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. |
| title_full_unstemmed | In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. |
| title_short | In vivo anti-tumor effects of Azadirachta indica in rat liver cancer. |
| title_sort | in vivo anti tumor effects of azadirachta indica in rat liver cancer |
| url | http://psasir.upm.edu.my/id/eprint/15176/1/In%20vivo%20anti.pdf |
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