Interfacial partition of plasmid DNA in aqueous two-phase systems
In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 °C) and mode of phase separation (40–60% (w/w) lysate concentrat...
| Հիմնական հեղինակներ: | , , |
|---|---|
| Ձևաչափ: | Հոդված |
| Լեզու: | English |
| Հրապարակվել է: |
Elsevier
2009
|
| Առցանց հասանելիություն: | http://psasir.upm.edu.my/id/eprint/15267/1/Interfacial%20partition%20of%20plasmid%20DNA%20in%20aqueous%20two-phase%20systems.pdf |
| _version_ | 1825945587851198464 |
|---|---|
| author | Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew |
| author_facet | Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew |
| author_sort | Luechau, Frank |
| collection | UPM |
| description | In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 °C) and mode of phase separation (40–60% (w/w) lysate concentrations) on the pDNA partition behaviour was studied. At 20 °C, 70.7% of pDNA partitioned to the top phase while the recoveries in the interface and bottom phase were comparatively small. At 30 °C, the partition had switched from a top phase preference to an interface preference where 73.9% of pDNA was recovered. The distribution of pDNA between the top phase and interface is effectively time-dependent during gravitational phase separation. Systems with lysate concentrations between 40 and 50% (w/w) separated with a continuous bottom phase and a dispersed PEG-rich top phase. For ATPS with 55 and 60% (w/w) lysate, the top phase was continuous and the bottom phase was dispersed. |
| first_indexed | 2024-03-06T07:33:50Z |
| format | Article |
| id | upm.eprints-15267 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:33:50Z |
| publishDate | 2009 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-152672019-05-09T01:28:26Z http://psasir.upm.edu.my/id/eprint/15267/ Interfacial partition of plasmid DNA in aqueous two-phase systems Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 °C) and mode of phase separation (40–60% (w/w) lysate concentrations) on the pDNA partition behaviour was studied. At 20 °C, 70.7% of pDNA partitioned to the top phase while the recoveries in the interface and bottom phase were comparatively small. At 30 °C, the partition had switched from a top phase preference to an interface preference where 73.9% of pDNA was recovered. The distribution of pDNA between the top phase and interface is effectively time-dependent during gravitational phase separation. Systems with lysate concentrations between 40 and 50% (w/w) separated with a continuous bottom phase and a dispersed PEG-rich top phase. For ATPS with 55 and 60% (w/w) lysate, the top phase was continuous and the bottom phase was dispersed. Elsevier 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15267/1/Interfacial%20partition%20of%20plasmid%20DNA%20in%20aqueous%20two-phase%20systems.pdf Luechau, Frank and Ling, Tau Chuan and Lyddiatt, Andrew (2009) Interfacial partition of plasmid DNA in aqueous two-phase systems. Separation and Purification Technology, 67 (1). pp. 64-70. ISSN 1383-5866; ESSN: 1873-3794 https://www.sciencedirect.com/science/article/pii/S1383586609001038#! 10.1016/j.seppur.2009.03.027 |
| spellingShingle | Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew Interfacial partition of plasmid DNA in aqueous two-phase systems |
| title | Interfacial partition of plasmid DNA in aqueous two-phase systems |
| title_full | Interfacial partition of plasmid DNA in aqueous two-phase systems |
| title_fullStr | Interfacial partition of plasmid DNA in aqueous two-phase systems |
| title_full_unstemmed | Interfacial partition of plasmid DNA in aqueous two-phase systems |
| title_short | Interfacial partition of plasmid DNA in aqueous two-phase systems |
| title_sort | interfacial partition of plasmid dna in aqueous two phase systems |
| url | http://psasir.upm.edu.my/id/eprint/15267/1/Interfacial%20partition%20of%20plasmid%20DNA%20in%20aqueous%20two-phase%20systems.pdf |
| work_keys_str_mv | AT luechaufrank interfacialpartitionofplasmiddnainaqueoustwophasesystems AT lingtauchuan interfacialpartitionofplasmiddnainaqueoustwophasesystems AT lyddiattandrew interfacialpartitionofplasmiddnainaqueoustwophasesystems |