Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii
Eight single locus microsatellite markers were developed to characterize the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. These microsatellites were isolated from an enriched genomic library contained by using a 5′‐anchored polymerase chain reaction technique. Primers were designed t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Blackwell Publishing
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15327/1/Isolation%20and%20characterization%20of%20microsatellite%20loci%20in%20the%20Malaysian%20giant%20freshwater%20prawn%2C%20Macrobrachium%20rosenbergii.pdf |
| _version_ | 1825945599393923072 |
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| author | Bhassu, Subha See, Leng Min Hassan, Rosly Siraj, Siti Shapor Tan, Soon Guan |
| author_facet | Bhassu, Subha See, Leng Min Hassan, Rosly Siraj, Siti Shapor Tan, Soon Guan |
| author_sort | Bhassu, Subha |
| collection | UPM |
| description | Eight single locus microsatellite markers were developed to characterize the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. These microsatellites were isolated from an enriched genomic library contained by using a 5′‐anchored polymerase chain reaction technique. Primers were designed to flank the repeat sequences and subsequently used to characterize 30 unrelated individuals of the giant freshwater prawn. The polymerase chain reaction amplification products of these eight microsatellite loci were polymorphic with the number of alleles ranging from two to 10 alleles per locus while the levels of heterozygosity ranged from 0.6333 to 0.8667. |
| first_indexed | 2024-03-06T07:34:01Z |
| format | Article |
| id | upm.eprints-15327 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:34:01Z |
| publishDate | 2008 |
| publisher | Blackwell Publishing |
| record_format | dspace |
| spelling | upm.eprints-153272019-07-08T07:28:27Z http://psasir.upm.edu.my/id/eprint/15327/ Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii Bhassu, Subha See, Leng Min Hassan, Rosly Siraj, Siti Shapor Tan, Soon Guan Eight single locus microsatellite markers were developed to characterize the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. These microsatellites were isolated from an enriched genomic library contained by using a 5′‐anchored polymerase chain reaction technique. Primers were designed to flank the repeat sequences and subsequently used to characterize 30 unrelated individuals of the giant freshwater prawn. The polymerase chain reaction amplification products of these eight microsatellite loci were polymorphic with the number of alleles ranging from two to 10 alleles per locus while the levels of heterozygosity ranged from 0.6333 to 0.8667. Blackwell Publishing 2008 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15327/1/Isolation%20and%20characterization%20of%20microsatellite%20loci%20in%20the%20Malaysian%20giant%20freshwater%20prawn%2C%20Macrobrachium%20rosenbergii.pdf Bhassu, Subha and See, Leng Min and Hassan, Rosly and Siraj, Siti Shapor and Tan, Soon Guan (2008) Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. Molecular Ecology Resources, 8 (5). pp. 983-985. ISSN 1755-098X; ESSN: 1755-0998 https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1755-0998.2008.02127.x 10.1111/j.1755-0998.2008.02127.x |
| spellingShingle | Bhassu, Subha See, Leng Min Hassan, Rosly Siraj, Siti Shapor Tan, Soon Guan Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii |
| title | Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii |
| title_full | Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii |
| title_fullStr | Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii |
| title_full_unstemmed | Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii |
| title_short | Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii |
| title_sort | isolation and characterization of microsatellite loci in the malaysian giant freshwater prawn macrobrachium rosenbergii |
| url | http://psasir.upm.edu.my/id/eprint/15327/1/Isolation%20and%20characterization%20of%20microsatellite%20loci%20in%20the%20Malaysian%20giant%20freshwater%20prawn%2C%20Macrobrachium%20rosenbergii.pdf |
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