Isozyme analysis and relationships among three species in Malaysian trichoderma isolates.
Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Thre...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2010
|
| Subjects: |
| _version_ | 1825945601794113536 |
|---|---|
| author | Siddique, Shafiquz zaman Tan, Soon Guan Yusuf, Umi Kalsom |
| author_facet | Siddique, Shafiquz zaman Tan, Soon Guan Yusuf, Umi Kalsom |
| author_sort | Siddique, Shafiquz zaman |
| collection | UPM |
| description | Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderma species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species. |
| first_indexed | 2024-03-06T07:34:03Z |
| format | Article |
| id | upm.eprints-15338 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:34:03Z |
| publishDate | 2010 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-153382012-07-23T04:38:05Z http://psasir.upm.edu.my/id/eprint/15338/ Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. Siddique, Shafiquz zaman Tan, Soon Guan Yusuf, Umi Kalsom Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderma species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species. Springer 2010 Article PeerReviewed Siddique, Shafiquz zaman and Tan, Soon Guan and Yusuf, Umi Kalsom (2010) Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. Journal of Microbiology and Biotechnology, 20 (9). pp. 1266-1275. ISSN 1017-7825 Isoenzymes. Enzymes - Analysis. Trichoderma. DOI: 10.4014/jmb.1004.04029 English |
| spellingShingle | Isoenzymes. Enzymes - Analysis. Trichoderma. Siddique, Shafiquz zaman Tan, Soon Guan Yusuf, Umi Kalsom Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. |
| title | Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. |
| title_full | Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. |
| title_fullStr | Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. |
| title_full_unstemmed | Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. |
| title_short | Isozyme analysis and relationships among three species in Malaysian trichoderma isolates. |
| title_sort | isozyme analysis and relationships among three species in malaysian trichoderma isolates |
| topic | Isoenzymes. Enzymes - Analysis. Trichoderma. |
| work_keys_str_mv | AT siddiqueshafiquzzaman isozymeanalysisandrelationshipsamongthreespeciesinmalaysiantrichodermaisolates AT tansoonguan isozymeanalysisandrelationshipsamongthreespeciesinmalaysiantrichodermaisolates AT yusufumikalsom isozymeanalysisandrelationshipsamongthreespeciesinmalaysiantrichodermaisolates |