LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth)
Solvent–solvent fractionation of the methanolic extract of the popular Malay traditional vegetable “tenggek burung” (Melicope ptelefolia), followed by nitric oxide inhibition assay on RAW 264.7 cells revealed that the most active components reside mainly in hexane and dichloromethane fractions. Onli...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Press
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15424/1/LC.pdf |
| _version_ | 1825945618484297728 |
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| author | Abas, Faridah Shaari, Khozirah Israf Ali, Daud Ahmad Syafri, Suryati Zainal, Zurina Lajis, Md. Nordin |
| author_facet | Abas, Faridah Shaari, Khozirah Israf Ali, Daud Ahmad Syafri, Suryati Zainal, Zurina Lajis, Md. Nordin |
| author_sort | Abas, Faridah |
| collection | UPM |
| description | Solvent–solvent fractionation of the methanolic extract of the popular Malay traditional vegetable “tenggek burung” (Melicope ptelefolia), followed by nitric oxide inhibition assay on RAW 264.7 cells revealed that the most active components reside mainly in hexane and dichloromethane fractions. Online profiling of the nitric oxide (NO) inhibitive fractions of the tenggek burung using liquid chromatography coupled diode array detection and electrospray ion-trap tandem mass spectroscopy (LC–DAD–ESI-MSn), has identified seven constituents. The compounds were identified as kokusaginine (1), compound 2, [kokusagine, (2a) or 5-methoxymaculine (2b)], 2,4,6-trihydroxy-3-prenylacetophenone (3), 2,4,6-trihydroxy-3-geranylacetophenone (4), 2,4,6-trihydroxy-3-geranylgeranylacetophenone (5), 3-[4-O-(3,7-dimethyl-2,6-octadienyl)phenyl]-2-propenoic acid (6) and 2,4,6-trihydroxy-3-farnesylgeranylacetophenone (7). The identity of compounds 1, 4 and 6 were unequivocally confirmed by isolation and spectroscopic evidences, other constituents are tentatively identified, based on their UV, MS, MSn and comparison with literature data. Kokusaginine (1) demonstrated in vitro activity on NO inhibition in murine peritoneal macrophages. |
| first_indexed | 2024-03-06T07:34:18Z |
| format | Article |
| id | upm.eprints-15424 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:34:18Z |
| publishDate | 2010 |
| publisher | Academic Press |
| record_format | dspace |
| spelling | upm.eprints-154242015-10-26T06:39:01Z http://psasir.upm.edu.my/id/eprint/15424/ LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) Abas, Faridah Shaari, Khozirah Israf Ali, Daud Ahmad Syafri, Suryati Zainal, Zurina Lajis, Md. Nordin Solvent–solvent fractionation of the methanolic extract of the popular Malay traditional vegetable “tenggek burung” (Melicope ptelefolia), followed by nitric oxide inhibition assay on RAW 264.7 cells revealed that the most active components reside mainly in hexane and dichloromethane fractions. Online profiling of the nitric oxide (NO) inhibitive fractions of the tenggek burung using liquid chromatography coupled diode array detection and electrospray ion-trap tandem mass spectroscopy (LC–DAD–ESI-MSn), has identified seven constituents. The compounds were identified as kokusaginine (1), compound 2, [kokusagine, (2a) or 5-methoxymaculine (2b)], 2,4,6-trihydroxy-3-prenylacetophenone (3), 2,4,6-trihydroxy-3-geranylacetophenone (4), 2,4,6-trihydroxy-3-geranylgeranylacetophenone (5), 3-[4-O-(3,7-dimethyl-2,6-octadienyl)phenyl]-2-propenoic acid (6) and 2,4,6-trihydroxy-3-farnesylgeranylacetophenone (7). The identity of compounds 1, 4 and 6 were unequivocally confirmed by isolation and spectroscopic evidences, other constituents are tentatively identified, based on their UV, MS, MSn and comparison with literature data. Kokusaginine (1) demonstrated in vitro activity on NO inhibition in murine peritoneal macrophages. Academic Press 2010-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15424/1/LC.pdf Abas, Faridah and Shaari, Khozirah and Israf Ali, Daud Ahmad and Syafri, Suryati and Zainal, Zurina and Lajis, Md. Nordin (2010) LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth). Journal of Food Composition and Analysis, 23 (1). pp. 107-112. ISSN 0889-1575; ESSN: 1096-0481 10.1016/j.jfca.2009.03.009 |
| spellingShingle | Abas, Faridah Shaari, Khozirah Israf Ali, Daud Ahmad Syafri, Suryati Zainal, Zurina Lajis, Md. Nordin LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) |
| title | LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) |
| title_full | LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) |
| title_fullStr | LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) |
| title_full_unstemmed | LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) |
| title_short | LC-DAD-ESI-MS analysis of nitric oxide inhibitory fractions of tenggek burung (Melicope ptelefolia Champ. ex Benth) |
| title_sort | lc dad esi ms analysis of nitric oxide inhibitory fractions of tenggek burung melicope ptelefolia champ ex benth |
| url | http://psasir.upm.edu.my/id/eprint/15424/1/LC.pdf |
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