Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies
A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier Ltd.
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15482/1/Localization%20of%20the%20antigenic%20sites%20of%20Newcastle%20disease%20virus%20nucleocapsid%20using%20a%20panel%20of%20monoclonal%20antibodies.pdf |
| _version_ | 1796968857500385280 |
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| author | Ahmad-Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Majid, Eshaghi Yusoff, Khatijah |
| author_facet | Ahmad-Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Majid, Eshaghi Yusoff, Khatijah |
| author_sort | Ahmad-Raus, Raha |
| collection | UPM |
| description | A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli.
One of the antigenic sites was located at the C-terminal end (residues 441 to 489) of the NP protein. Two other antigenic sites were located within the N-terminal end (residues 26–121 and 122–375). This study demonstrates that the N- and C-terminal ends of the NP proteins are responsible in eliciting immune response, thus it is most likely that these ends are exposed on the NP. |
| first_indexed | 2024-03-06T07:34:28Z |
| format | Article |
| id | upm.eprints-15482 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:34:28Z |
| publishDate | 2009 |
| publisher | Elsevier Ltd. |
| record_format | dspace |
| spelling | upm.eprints-154822015-11-24T06:37:13Z http://psasir.upm.edu.my/id/eprint/15482/ Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies Ahmad-Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Majid, Eshaghi Yusoff, Khatijah A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located at the C-terminal end (residues 441 to 489) of the NP protein. Two other antigenic sites were located within the N-terminal end (residues 26–121 and 122–375). This study demonstrates that the N- and C-terminal ends of the NP proteins are responsible in eliciting immune response, thus it is most likely that these ends are exposed on the NP. Elsevier Ltd. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15482/1/Localization%20of%20the%20antigenic%20sites%20of%20Newcastle%20disease%20virus%20nucleocapsid%20using%20a%20panel%20of%20monoclonal%20antibodies.pdf Ahmad-Raus, Raha and Ali, Abdul Manaf and Tan, Wen Siang and Mohd. Salleh, Hamzah and Majid, Eshaghi and Yusoff, Khatijah (2009) Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies. Research in Veterinary Science, 86 (1). pp. 174-182. ISSN 0034-5288 Monoclonal antibody Antibodies, Monoclonal - Analysis Newcastle disease virus doi:10.1016/j.rvsc.2008.05.013 English |
| spellingShingle | Monoclonal antibody Antibodies, Monoclonal - Analysis Newcastle disease virus Ahmad-Raus, Raha Ali, Abdul Manaf Tan, Wen Siang Mohd. Salleh, Hamzah Majid, Eshaghi Yusoff, Khatijah Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| title | Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| title_full | Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| title_fullStr | Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| title_full_unstemmed | Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| title_short | Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| title_sort | localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies |
| topic | Monoclonal antibody Antibodies, Monoclonal - Analysis Newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/15482/1/Localization%20of%20the%20antigenic%20sites%20of%20Newcastle%20disease%20virus%20nucleocapsid%20using%20a%20panel%20of%20monoclonal%20antibodies.pdf |
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