Medium optimization for chitinase production from Trichoderma virens using central composite design
Medium development for chitinase production by Trichoderma virens was first carried out using conventional method of one-factor-at-a-time. The medium was further optimized using Central Composite Design in which response surface was generated later from the derived model. An experimental design of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Science+Business Media
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/15599/1/Medium%20optimization%20for%20chitinase%20production%20from%20Trichoderma%20virens%20using%20central%20composite%20design.pdf |
| _version_ | 1825945652171898880 |
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| author | Wasli, Azaliza Safarida Md. Salleh, Madihah Abd. Aziz , Suraini Hassan , Osmanan Mahadi, Nor Muhammad |
| author_facet | Wasli, Azaliza Safarida Md. Salleh, Madihah Abd. Aziz , Suraini Hassan , Osmanan Mahadi, Nor Muhammad |
| author_sort | Wasli, Azaliza Safarida |
| collection | UPM |
| description | Medium development for chitinase production by Trichoderma virens was first carried out using conventional method
of one-factor-at-a-time. The medium was further optimized using Central Composite Design in which response surface
was generated later from the derived model. An experimental design of four variables including various initial pH
values, chitin, ammonium sulphate, and methanol concentrations were created using Design Expert®
Software, Version 6.0. The design consists of 30 experiments, which include 6 replicates at center points. The optimal value for each variable are 3.0 g/L, chitin; 0.1 g/L, ammonium sulphate; 0.4% (v/v), methanol; and initial pH, 4.0 with predicted chitinase activity of 0.1495 U/mL. These predicted parameters were tested in the laboratory and the final chitinase activity obtained was 0.1471 U/mL, which is almost reaching the predicted value. The optimal medium design showed an improvement of chitinase activity of 80.9% compared to activity obtained from the original Absidia medium composition. |
| first_indexed | 2024-03-06T07:34:49Z |
| format | Article |
| id | upm.eprints-15599 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:34:49Z |
| publishDate | 2009 |
| publisher | Springer Science+Business Media |
| record_format | dspace |
| spelling | upm.eprints-155992015-10-22T01:10:40Z http://psasir.upm.edu.my/id/eprint/15599/ Medium optimization for chitinase production from Trichoderma virens using central composite design Wasli, Azaliza Safarida Md. Salleh, Madihah Abd. Aziz , Suraini Hassan , Osmanan Mahadi, Nor Muhammad Medium development for chitinase production by Trichoderma virens was first carried out using conventional method of one-factor-at-a-time. The medium was further optimized using Central Composite Design in which response surface was generated later from the derived model. An experimental design of four variables including various initial pH values, chitin, ammonium sulphate, and methanol concentrations were created using Design Expert® Software, Version 6.0. The design consists of 30 experiments, which include 6 replicates at center points. The optimal value for each variable are 3.0 g/L, chitin; 0.1 g/L, ammonium sulphate; 0.4% (v/v), methanol; and initial pH, 4.0 with predicted chitinase activity of 0.1495 U/mL. These predicted parameters were tested in the laboratory and the final chitinase activity obtained was 0.1471 U/mL, which is almost reaching the predicted value. The optimal medium design showed an improvement of chitinase activity of 80.9% compared to activity obtained from the original Absidia medium composition. Springer Science+Business Media 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15599/1/Medium%20optimization%20for%20chitinase%20production%20from%20Trichoderma%20virens%20using%20central%20composite%20design.pdf Wasli, Azaliza Safarida and Md. Salleh, Madihah and Abd. Aziz , Suraini and Hassan , Osmanan and Mahadi, Nor Muhammad (2009) Medium optimization for chitinase production from Trichoderma virens using central composite design. Biotechnology and Bioprocess Engineering, 14 (1). pp. 781-787. ISSN 1226-8372 Chitinase Trichoderma Experimental design DOI: 10.1007/s12257-008-0127-z English |
| spellingShingle | Chitinase Trichoderma Experimental design Wasli, Azaliza Safarida Md. Salleh, Madihah Abd. Aziz , Suraini Hassan , Osmanan Mahadi, Nor Muhammad Medium optimization for chitinase production from Trichoderma virens using central composite design |
| title | Medium optimization for chitinase production from Trichoderma virens using central composite design |
| title_full | Medium optimization for chitinase production from Trichoderma virens using central composite design |
| title_fullStr | Medium optimization for chitinase production from Trichoderma virens using central composite design |
| title_full_unstemmed | Medium optimization for chitinase production from Trichoderma virens using central composite design |
| title_short | Medium optimization for chitinase production from Trichoderma virens using central composite design |
| title_sort | medium optimization for chitinase production from trichoderma virens using central composite design |
| topic | Chitinase Trichoderma Experimental design |
| url | http://psasir.upm.edu.my/id/eprint/15599/1/Medium%20optimization%20for%20chitinase%20production%20from%20Trichoderma%20virens%20using%20central%20composite%20design.pdf |
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