Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia
In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I(COI) gene. In total, 92 T. tambroides samples were analyzed from 4 loc...
| Autori principali: | , , , , , |
|---|---|
| Natura: | Articolo |
| Lingua: | English |
| Pubblicazione: |
Biodiversity Research Center, Academia Sinica, Taiwan
2008
|
| Accesso online: | http://psasir.upm.edu.my/id/eprint/15645/1/Mitochondrial%20DNA%20diversity%20of%20Tor%20douronensis%20Valenciennes.pdf |
| _version_ | 1825945661242081280 |
|---|---|
| author | Esa, Yuzine Siraj, Siti Shapor Daud, Siti Khalijah A. Rahim, Khairul Adha Japning, Jeffrine Rovie Ryan Tan, Soon Guan |
| author_facet | Esa, Yuzine Siraj, Siti Shapor Daud, Siti Khalijah A. Rahim, Khairul Adha Japning, Jeffrine Rovie Ryan Tan, Soon Guan |
| author_sort | Esa, Yuzine |
| collection | UPM |
| description | In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater
fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I(COI) gene. In total, 92 T. tambroides samples were analyzed from 4 locations on Peninsular Malaysia (n=87) and a single
population from Sarawak (Batang Ai, n=5) on Borneo Island, and 4 sequences of Tor douronensis from Sarawak were used for comparisons. In total, 9 haplotypes were found, with 7 haplotypes being unique and 2 haplotypes being shared among the 5 populations. The phylogenetic analysis using Neighbor-joining (NJ) and maximum-parsimony (MP) methods supported
the monophyletic status between T. tambroides and T. douronensis, thus suggesting their status as different species. The clustering of all T. tambroides samples into a single clade suggested that their genetic identity belongs to a single species. The sharing of haplotype HKE4 between Batang Ai of Sarawak (n= 4) and Perak of Peninsular Malaysia (n= 3) reflects the historical connection of drainages between the regions possibly during Pleistocene glaciation periods. Limited variations were found among all peninsular T. tambroides populations. The low level of mitochondrial (mt)DNA differences currently found among
T. tambroides populations is probably due to the high proportion of the HKE1 haplotype being found in all the populations (0.736-1.000), or the small number of samples used in the present study. Overall, the present study was able to shed light on the phylogenetic relationships
and genetic structure of T. tambroides in Malaysia. |
| first_indexed | 2024-03-06T07:34:57Z |
| format | Article |
| id | upm.eprints-15645 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:34:57Z |
| publishDate | 2008 |
| publisher | Biodiversity Research Center, Academia Sinica, Taiwan |
| record_format | dspace |
| spelling | upm.eprints-156452016-05-16T05:03:06Z http://psasir.upm.edu.my/id/eprint/15645/ Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia Esa, Yuzine Siraj, Siti Shapor Daud, Siti Khalijah A. Rahim, Khairul Adha Japning, Jeffrine Rovie Ryan Tan, Soon Guan In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I(COI) gene. In total, 92 T. tambroides samples were analyzed from 4 locations on Peninsular Malaysia (n=87) and a single population from Sarawak (Batang Ai, n=5) on Borneo Island, and 4 sequences of Tor douronensis from Sarawak were used for comparisons. In total, 9 haplotypes were found, with 7 haplotypes being unique and 2 haplotypes being shared among the 5 populations. The phylogenetic analysis using Neighbor-joining (NJ) and maximum-parsimony (MP) methods supported the monophyletic status between T. tambroides and T. douronensis, thus suggesting their status as different species. The clustering of all T. tambroides samples into a single clade suggested that their genetic identity belongs to a single species. The sharing of haplotype HKE4 between Batang Ai of Sarawak (n= 4) and Perak of Peninsular Malaysia (n= 3) reflects the historical connection of drainages between the regions possibly during Pleistocene glaciation periods. Limited variations were found among all peninsular T. tambroides populations. The low level of mitochondrial (mt)DNA differences currently found among T. tambroides populations is probably due to the high proportion of the HKE1 haplotype being found in all the populations (0.736-1.000), or the small number of samples used in the present study. Overall, the present study was able to shed light on the phylogenetic relationships and genetic structure of T. tambroides in Malaysia. Biodiversity Research Center, Academia Sinica, Taiwan 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15645/1/Mitochondrial%20DNA%20diversity%20of%20Tor%20douronensis%20Valenciennes.pdf Esa, Yuzine and Siraj, Siti Shapor and Daud, Siti Khalijah and A. Rahim, Khairul Adha and Japning, Jeffrine Rovie Ryan and Tan, Soon Guan (2008) Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia. Zoological Studies, 47 (3). pp. 360-367. ISSN 1021-5506 http://zoolstud.sinica.edu.tw/473.htm |
| spellingShingle | Esa, Yuzine Siraj, Siti Shapor Daud, Siti Khalijah A. Rahim, Khairul Adha Japning, Jeffrine Rovie Ryan Tan, Soon Guan Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia |
| title | Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia |
| title_full | Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia |
| title_fullStr | Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia |
| title_full_unstemmed | Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia |
| title_short | Mitochondrial DNA diversity of Tor douronensis Valenciennes (Cyprinidae) from five natural populations in Malaysia |
| title_sort | mitochondrial dna diversity of tor douronensis valenciennes cyprinidae from five natural populations in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/15645/1/Mitochondrial%20DNA%20diversity%20of%20Tor%20douronensis%20Valenciennes.pdf |
| work_keys_str_mv | AT esayuzine mitochondrialdnadiversityoftordouronensisvalenciennescyprinidaefromfivenaturalpopulationsinmalaysia AT sirajsitishapor mitochondrialdnadiversityoftordouronensisvalenciennescyprinidaefromfivenaturalpopulationsinmalaysia AT daudsitikhalijah mitochondrialdnadiversityoftordouronensisvalenciennescyprinidaefromfivenaturalpopulationsinmalaysia AT arahimkhairuladha mitochondrialdnadiversityoftordouronensisvalenciennescyprinidaefromfivenaturalpopulationsinmalaysia AT japningjeffrinerovieryan mitochondrialdnadiversityoftordouronensisvalenciennescyprinidaefromfivenaturalpopulationsinmalaysia AT tansoonguan mitochondrialdnadiversityoftordouronensisvalenciennescyprinidaefromfivenaturalpopulationsinmalaysia |